Polymorphic Protective Dps-DNA Co-Crystals by Cryo Electron Tomography and Small Angle X-Ray Scattering.

Amino Acid Sequence Bacterial Outer Membrane Proteins / chemistry Crystallography, X-Ray DNA / chemistry Electron Microscope Tomography Models, Molecular Nucleic Acid Conformation Protein Conformation Scattering, Small Angle
DNA Dps biocrystallization co-crystals cryo-electron tomography small-angle X-ray scattering

Journal

Biomolecules
ISSN: 2218-273X
Titre abrégé: Biomolecules
Pays: Switzerland
ID NLM: 101596414

Informations de publication

Date de publication:
26 12 2019
Historique:
received: 06 11 2019
revised: 19 12 2019
accepted: 22 12 2019
entrez: 1 1 2020
pubmed: 1 1 2020
medline: 17 9 2020
Statut: epublish

Résumé

Rapid increase of intracellular synthesis of specific histone-like Dps protein that binds DNA to protect the genome against deleterious factors leads to in cellulo crystallization-one of the most curious processes in the area of life science at the moment. However, the actual structure of the Dps-DNA co-crystals remained uncertain in the details for more than two decades. Cryo-electron tomography and small-angle X-ray scattering revealed polymorphous modifications of the co-crystals depending on the buffer parameters. Two different types of the Dps-DNA co-crystals are formed in vitro: triclinic and cubic. Three-dimensional reconstruction revealed DNA and Dps molecules in cubic co-crystals, and the unit cell parameters of cubic lattice were determined consistently by both methods.

Identifiants

DOI: 10.3390/biom10010039 PMID: 31888079 PMC: PMC7023142
pubmed: 31888079
pii: biom10010039
doi: 10.3390/biom10010039
pmc: PMC7023142
pii:
doi:

Substances chimiques

Bacterial Outer Membrane Proteins 0
DNA 9007-49-2

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

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Auteurs

Roman Kamyshinsky (R)

National Research Center "Kurchatov Institute", Akademika Kurchatova pl., 1, 123182 Moscow, Russia.
Shubnikov Institute of Crystallography of Federal Scientific Research Centre "Crystallography and Photonics" of Russian Academy of Sciences, Leninskiy prospect, 59, 119333 Moscow, Russia.
Moscow Institute of Physics and Technology, Institutsky lane 9, 141700 Dolgoprudny, Moscow Region, Russia.
ORCID: 0000-0002-8546-7763

Yury Chesnokov (Y)

National Research Center "Kurchatov Institute", Akademika Kurchatova pl., 1, 123182 Moscow, Russia.
Shubnikov Institute of Crystallography of Federal Scientific Research Centre "Crystallography and Photonics" of Russian Academy of Sciences, Leninskiy prospect, 59, 119333 Moscow, Russia.
ORCID: 0000-0001-8365-4699

Liubov Dadinova (L)

Shubnikov Institute of Crystallography of Federal Scientific Research Centre "Crystallography and Photonics" of Russian Academy of Sciences, Leninskiy prospect, 59, 119333 Moscow, Russia.

Andrey Mozhaev (A)

Shubnikov Institute of Crystallography of Federal Scientific Research Centre "Crystallography and Photonics" of Russian Academy of Sciences, Leninskiy prospect, 59, 119333 Moscow, Russia.
Shemyakin-Ovchinnikov Institute of bioorganic chemistry of Russian Academy of Sciences, Miklukho-Maklaya, 16/10, 117997 Moscow, Russia.

Ivan Orlov (I)

Shubnikov Institute of Crystallography of Federal Scientific Research Centre "Crystallography and Photonics" of Russian Academy of Sciences, Leninskiy prospect, 59, 119333 Moscow, Russia.

Maxim Petoukhov (M)

Shubnikov Institute of Crystallography of Federal Scientific Research Centre "Crystallography and Photonics" of Russian Academy of Sciences, Leninskiy prospect, 59, 119333 Moscow, Russia.
Frumkin Institute of Physical Chemistry and Electrochemistry of Russian Academy of Sciences, Leninsky prospect, 31, 119071 Moscow, Russia.
ORCID: 0000-0001-6760-4595

Anton Orekhov (A)

National Research Center "Kurchatov Institute", Akademika Kurchatova pl., 1, 123182 Moscow, Russia.
Shubnikov Institute of Crystallography of Federal Scientific Research Centre "Crystallography and Photonics" of Russian Academy of Sciences, Leninskiy prospect, 59, 119333 Moscow, Russia.
Moscow Institute of Physics and Technology, Institutsky lane 9, 141700 Dolgoprudny, Moscow Region, Russia.

Eleonora Shtykova (E)

Shubnikov Institute of Crystallography of Federal Scientific Research Centre "Crystallography and Photonics" of Russian Academy of Sciences, Leninskiy prospect, 59, 119333 Moscow, Russia.

Alexander Vasiliev (A)

National Research Center "Kurchatov Institute", Akademika Kurchatova pl., 1, 123182 Moscow, Russia.
Shubnikov Institute of Crystallography of Federal Scientific Research Centre "Crystallography and Photonics" of Russian Academy of Sciences, Leninskiy prospect, 59, 119333 Moscow, Russia.
Moscow Institute of Physics and Technology, Institutsky lane 9, 141700 Dolgoprudny, Moscow Region, Russia.

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Classifications MeSH

questionsmedicales.fr › Sciences de l'information › Sources d'information › Services d'information › Documentation › Données de séquences moléculaires › Séquence d'acides aminés › Polymorphic Protective Dps-DNA Co-Crystals by...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines membranaires › Protéines de la membrane externe bactérienne › Polymorphic Protective Dps-DNA Co-Crystals by...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Cristallographie aux rayons X › Polymorphic Protective Dps-DNA Co-Crystals by...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ADN › Polymorphic Protective Dps-DNA Co-Crystals by...
questionsmedicales.fr › Techniques d'investigation › Microscopie électronique › Microscopie électronique à transmission › Tomographie en microscopie électronique › Polymorphic Protective Dps-DNA Co-Crystals by...
questionsmedicales.fr › Techniques d'investigation › Modèles théoriques › Modèles moléculaires › Polymorphic Protective Dps-DNA Co-Crystals by...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Conformation d'acide nucléique › Polymorphic Protective Dps-DNA Co-Crystals by...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Conformation moléculaire › Conformation des protéines › Polymorphic Protective Dps-DNA Co-Crystals by...
questionsmedicales.fr › Phénomènes physiques › Diffusion de rayonnements › Diffusion aux petits angles › Polymorphic Protective Dps-DNA Co-Crystals by...