Chicken seminal fluid lacks CD9- and CD44-bearing extracellular vesicles.
CD44
CD9
electron microscopy
exosome precipitation
rooster
seminal fluid
tetraspanins
western blot
Journal
Reproduction in domestic animals = Zuchthygiene
ISSN: 1439-0531
Titre abrégé: Reprod Domest Anim
Pays: Germany
ID NLM: 9015668
Informations de publication
Date de publication:
Mar 2020
Mar 2020
Historique:
received:
11
11
2019
accepted:
26
12
2019
pubmed:
3
1
2020
medline:
7
10
2020
entrez:
3
1
2020
Statut:
ppublish
Résumé
The avian seminal fluid (SF) is a protein-rich fluid, derived from the testis, the rudimentary epididymis and, finally, from the cloacal gland. The SF interacts with spermatozoa and the inner cell lining of the female genital tract, to modulate sperm functions and female immune responsiveness. Its complex proteome might either be free or linked to extracellular vesicles (EVs) as it is the case in mammals, where EVs depict the tetraspanin CD9; and where those EVs derived from the epididymis (epididymosomes) also present the receptor CD44. In the present study, sperm-free SF from Red Jungle Fowl, White Leghorn and an advanced intercross (AIL, 12th generation) were studied using flow cytometry of the membrane marker tetraspanin CD9, Western blotting of the membrane receptor CD44 and electron microscopy in non-enriched (whole SF) or enriched fractions obtained by precipitation using a commercial kit (Total Exosome Precipitation Solution). Neither CD9- nor CD44 could be detected, and the ultrastructure confirmed the relative absence of EVs, raising the possibility that avian SF interacts differently with the female genitalia as compared to the seminal plasma of mammals.
Substances chimiques
Hyaluronan Receptors
0
Tetraspanin 29
0
Types de publication
Comparative Study
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
293-300Subventions
Organisme : Vetenskapsrådet
ID : 2015-05919
Organisme : Forskningsrådet i Sydöstra Sverige
ID : 473121
Organisme : Forskningsrådet i Sydöstra Sverige
ID : 745971
Organisme : Lions Forskningsfond
ID : DNR LIU-2016-00641
Organisme : Swedish Research Council FORMAS
ID : 2017-00946
Informations de copyright
© 2020 Blackwell Verlag GmbH.
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