The pitfalls and promise of liquid biopsies for diagnosing and treating solid tumors in children: a review.


Journal

European journal of pediatrics
ISSN: 1432-1076
Titre abrégé: Eur J Pediatr
Pays: Germany
ID NLM: 7603873

Informations de publication

Date de publication:
Feb 2020
Historique:
received: 29 09 2019
accepted: 04 12 2019
revised: 29 11 2019
pubmed: 4 1 2020
medline: 20 11 2020
entrez: 4 1 2020
Statut: ppublish

Résumé

Cell-free DNA profiling using patient blood is emerging as a non-invasive complementary technique for cancer genomic characterization. Since these liquid biopsies will soon be integrated into clinical trial protocols for pediatric cancer treatment, clinicians should be informed about potential applications and advantages but also weaknesses and potential pitfalls. Small retrospective studies comparing genetic alterations detected in liquid biopsies with tumor biopsies for pediatric solid tumor types are encouraging. Molecular detection of tumor markers in cell-free DNA could be used for earlier therapy response monitoring and residual disease detection as well as enabling detection of pathognomonic and therapeutically relevant genomic alterations.Conclusion: Existing analyses of liquid biopsies from children with solid tumors increasingly suggest a potential relevance for molecular diagnostics, prognostic assessment, and therapeutic decision-making. Gaps remain in the types of tumors studied and value of detection methods applied. Here we review the current stand of liquid biopsy studies for pediatric solid tumors with a dedicated focus on cell-free DNA analysis. There is legitimate hope that integrating fully validated liquid biopsy-based innovations into the standard of care will advance patient monitoring and personalized treatment of children battling solid cancers.What is Known:• Liquid biopsies are finding their way into routine oncological screening, diagnosis, and disease monitoring in adult cancer types fast.• The most widely adopted source for liquid biopsies is blood although other easily accessible body fluids, such as saliva, pleural effusions, urine, or cerebrospinal fluid (CSF) can also serve as sources for liquid biopsiesWhat is New:• Retrospective proof-of-concept studies in small cohorts illustrate that liquid biopsies in pediatric solid tumors yield tremendous potential to be used in diagnostics, for therapy response monitoring and in residual disease detection.• Liquid biopsy diagnostics could tackle some long-standing issues in the pediatric oncology field; they can enable accurate genetic diagnostics in previously unbiopsied tumor types like renal tumors or brain stem tumors leading to better treatment strategies.

Identifiants

pubmed: 31897843
doi: 10.1007/s00431-019-03545-y
pii: 10.1007/s00431-019-03545-y
pmc: PMC6971142
doi:

Types de publication

Journal Article Review

Langues

eng

Sous-ensembles de citation

IM

Pagination

191-202

Commentaires et corrections

Type : ErratumIn

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Auteurs

Ruben Van Paemel (R)

Center for Medical Genetics, Ghent University Hospital, Ghent, Belgium.
Cancer Research Institute Ghent (CRIG), Ghent, Belgium.
Department of Pediatric Hematology, Oncology and Stem Cell Transplantation, Ghent University Hospital, C. Heymanslaan 10, 9000, Ghent, Belgium.

Roos Vlug (R)

Department of Pediatric Hematology, Oncology and Stem Cell Transplantation, Ghent University Hospital, C. Heymanslaan 10, 9000, Ghent, Belgium.

Katleen De Preter (K)

Center for Medical Genetics, Ghent University Hospital, Ghent, Belgium.
Cancer Research Institute Ghent (CRIG), Ghent, Belgium.

Nadine Van Roy (N)

Center for Medical Genetics, Ghent University Hospital, Ghent, Belgium.
Cancer Research Institute Ghent (CRIG), Ghent, Belgium.

Frank Speleman (F)

Center for Medical Genetics, Ghent University Hospital, Ghent, Belgium.
Cancer Research Institute Ghent (CRIG), Ghent, Belgium.

Leen Willems (L)

Department of Pediatric Hematology, Oncology and Stem Cell Transplantation, Ghent University Hospital, C. Heymanslaan 10, 9000, Ghent, Belgium.

Tim Lammens (T)

Cancer Research Institute Ghent (CRIG), Ghent, Belgium.
Department of Pediatric Hematology, Oncology and Stem Cell Transplantation, Ghent University Hospital, C. Heymanslaan 10, 9000, Ghent, Belgium.

Geneviève Laureys (G)

Cancer Research Institute Ghent (CRIG), Ghent, Belgium.
Department of Pediatric Hematology, Oncology and Stem Cell Transplantation, Ghent University Hospital, C. Heymanslaan 10, 9000, Ghent, Belgium.

Gudrun Schleiermacher (G)

INSERM U830, Laboratoire de Genetique et Biologie des Cancers, Research Center, PSL Research University, Institut Curie, Paris, France.
SiRIC RTOP «Recherche Translationelle en Oncologie Pediatrique », Translational Research Department, Research Center, PSL Research University, Institut Curie, Paris, France.
Department of Pediatric Oncology, Hospital Group, Institut Curie, Paris, France.

Godelieve A M Tytgat (GAM)

Princess Máxima Center for Pediatric Oncology, Utrecht, The Netherlands.

Kathy Astrahantseff (K)

Department of Pediatric Hematology and Oncology, Charité - Universitätsmedizin Berlin, Berlin, Germany.

Hedwig Deubzer (H)

Department of Pediatric Hematology and Oncology, Charité - Universitätsmedizin Berlin, Berlin, Germany.
Berlin Institute of Health (BIH), Berlin, Germany.
Neuroblastoma Research Group, Experimental and Clinical Research Center (ECRC), Berlin, Germany.

Bram De Wilde (B)

Center for Medical Genetics, Ghent University Hospital, Ghent, Belgium. Bram.DeWilde@uzgent.be.
Cancer Research Institute Ghent (CRIG), Ghent, Belgium. Bram.DeWilde@uzgent.be.
Department of Pediatric Hematology, Oncology and Stem Cell Transplantation, Ghent University Hospital, C. Heymanslaan 10, 9000, Ghent, Belgium. Bram.DeWilde@uzgent.be.

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