Bacterial growth, morphology, and cell component changes in Herbaspirillum sp. WT00C exposed to high concentration of selenate.
Herbaspirillum
oxidative stress
proteomic analysis
scanning electron microscopy
selenate
Journal
Journal of basic microbiology
ISSN: 1521-4028
Titre abrégé: J Basic Microbiol
Pays: Germany
ID NLM: 8503885
Informations de publication
Date de publication:
Apr 2020
Apr 2020
Historique:
received:
28
10
2019
revised:
29
11
2019
accepted:
08
12
2019
pubmed:
4
1
2020
medline:
9
10
2020
entrez:
4
1
2020
Statut:
ppublish
Résumé
Selenium (Se) is a nonmetallic element of the chalcogens. It is primarily available in natural environments as selenate and selenite oxoanions. Although selenate/selenite reduction in many microbes is widely studied at low concentrations (<50 mM), the effects of high selenate stress on bacterial growth, morphology, and cell components have not yet been studied. In this study, the response of Herbaspirillum sp. WT00C to selenate stress at high concentration is investigated by microbiological and scanning electron microscopy (SEM) techniques as well as proteomic analysis. Bacterial growth was seriously inhibited under high selenate concentrations and its growth-inhibitory phase was prolonged with the increase of selenate concentrations. More interestingly, this bacterium was able to recover its growth even if the selenate concentration was up to 400 mM. Its growth inhibition period shortened to 6 h when the bacterium growing in 200 mM selenate for 28 h was reinoculated to the Luria-Bertani medium containing 200 mM selenate. The high concentration of selenate also induces marked changes in the cell dimension and surface roughness, as revealed by SEM, along with compositional changes in the cell wall shown by proteomic analysis. The bacterial growth inhibition results from the marked downregulation of the α-subunit of DNA polymerase III and RNA helicase, whereas its growth recovery is related to its high antioxidative activities. More NADPH synthesis and the upregulation of thioredoxin reductase and GPx are beneficial for Herbaspirillum sp. WT00C to establish and maintain a balance between oxidant and antioxidant intracellular systems for defending selenate toxicity. This study is an important contribution to understanding why Herbaspirillum sp. WT00C survives in a high concentration of selenate and how the bacterial cells respond physiologically to selenate stress at high concentration.
Identifiants
pubmed: 31898337
doi: 10.1002/jobm.201900586
doi:
Substances chimiques
Antioxidants
0
Selenious Acid
F6A27P4Q4R
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
304-321Subventions
Organisme : Ministry of Science and Technology of the People's Republic of China
ID : 2016YFD0200905
Informations de copyright
© 2020 The Authors. Journal of Basic Microbiology published by Wiley-VCH Verlag GmbH Co. KGaA, Weinheim& Co. KGaA.
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