Proteomic analysis identified proteins that are differentially expressed in the flavonoid and carotenoid biosynthetic pathways of Camellia Nitidissima flowers.
Camellia Nitidissima
Flower color
Metabolic pathway
Metabolites
Proteome
Tandem mass tag (TMT)
Journal
BMC plant biology
ISSN: 1471-2229
Titre abrégé: BMC Plant Biol
Pays: England
ID NLM: 100967807
Informations de publication
Date de publication:
01 Nov 2024
01 Nov 2024
Historique:
received:
23
10
2023
accepted:
21
10
2024
medline:
1
11
2024
pubmed:
1
11
2024
entrez:
1
11
2024
Statut:
epublish
Résumé
Camellia nitidissima Chi is a popular ornamental plant because of its golden flowers, which contain flavonoids and carotenoids. To understand the regulatory mechanism of golden color formation, the metabolites of C. nitidissima petals at five different developmental stages were detected, a proteome map of petals was first constructed via tandem mass tag (TMT) analysis, and the accuracy of the sequencing data was validated via parallel reaction monitoring (PRM). Nineteen color components were detected, and most of these components were carotenoids that gradually accumulated, while some metabolites were flavonoids that were gradually depleted. A total of 97,647 spectra were obtained, and 6,789 quantifiable proteins were identified. Then, 1,319 differentially expressed proteins (DEPs) were found, 55 of which belong to the flavonoid and carotenoid pathways, as revealed by pairwise comparisons of protein expression levels across the five developmental stages. Notably, most DEPs involved in the synthesis of flavonoids, such as phenylalanine ammonium lyase and 4-coumarate-CoA ligase, were downregulated during petal development, whereas DEPs involved in carotenoid synthesis, such as phytoene synthase, 1-deoxy-D-xylulose-5-phosphate synthase, and β-cyclase, tended to be upregulated. Furthermore, protein‒protein interaction (PPI) network analysis revealed that these 55 DEPs formed two distinct PPI networks closely tied to the flavonoid and carotenoid synthesis pathways. Phytoene synthase and chalcone synthase exhibited extensive interactions with numerous other proteins and displayed high connectivity within the PPI networks, suggesting their pivotal biological functions in flavonoid and carotenoid biosynthesis. Proteomic data on the flavonoid and carotenoid biosynthesis pathways were obtained, and the regulatory roles of the DEPs were analyzed, which provided a theoretical basis for further understanding the golden color formation mechanism of C. nitidissima.
Sections du résumé
BACKGROUND
BACKGROUND
Camellia nitidissima Chi is a popular ornamental plant because of its golden flowers, which contain flavonoids and carotenoids. To understand the regulatory mechanism of golden color formation, the metabolites of C. nitidissima petals at five different developmental stages were detected, a proteome map of petals was first constructed via tandem mass tag (TMT) analysis, and the accuracy of the sequencing data was validated via parallel reaction monitoring (PRM).
RESULTS
RESULTS
Nineteen color components were detected, and most of these components were carotenoids that gradually accumulated, while some metabolites were flavonoids that were gradually depleted. A total of 97,647 spectra were obtained, and 6,789 quantifiable proteins were identified. Then, 1,319 differentially expressed proteins (DEPs) were found, 55 of which belong to the flavonoid and carotenoid pathways, as revealed by pairwise comparisons of protein expression levels across the five developmental stages. Notably, most DEPs involved in the synthesis of flavonoids, such as phenylalanine ammonium lyase and 4-coumarate-CoA ligase, were downregulated during petal development, whereas DEPs involved in carotenoid synthesis, such as phytoene synthase, 1-deoxy-D-xylulose-5-phosphate synthase, and β-cyclase, tended to be upregulated. Furthermore, protein‒protein interaction (PPI) network analysis revealed that these 55 DEPs formed two distinct PPI networks closely tied to the flavonoid and carotenoid synthesis pathways. Phytoene synthase and chalcone synthase exhibited extensive interactions with numerous other proteins and displayed high connectivity within the PPI networks, suggesting their pivotal biological functions in flavonoid and carotenoid biosynthesis.
CONCLUSION
CONCLUSIONS
Proteomic data on the flavonoid and carotenoid biosynthesis pathways were obtained, and the regulatory roles of the DEPs were analyzed, which provided a theoretical basis for further understanding the golden color formation mechanism of C. nitidissima.
Identifiants
pubmed: 39482574
doi: 10.1186/s12870-024-05737-7
pii: 10.1186/s12870-024-05737-7
doi:
Substances chimiques
Carotenoids
36-88-4
Plant Proteins
0
Flavonoids
0
Proteome
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1037Subventions
Organisme : the Key Program of Guangxi Nature Science Foundation
ID : 2018GXNSFDA281007
Organisme : the Key Program of Guangxi Nature Science Foundation
ID : 2018GXNSFDA281007
Organisme : the scientific research foundation project of Fujian University of Technology
ID : GY-Z22041
Organisme : Fujian forestry science and technology project
ID : ZMGG-0702
Organisme : Fujian forestry science and technology project
ID : ZMGG-0702
Organisme : Foundation for Innovative Research Groups of the National Natural Science Foundation of China
ID : 31860228
Organisme : Foundation for Innovative Research Groups of the National Natural Science Foundation of China
ID : 31860228
Organisme : Foundation for Innovative Research Groups of the National Natural Science Foundation of China
ID : 31860228
Organisme : the project of scientific research foundation in Yulin Normal University
ID : G2019ZK13, G2019ZK35
Organisme : the project of scientific research foundation in Yulin Normal University
ID : G2019ZK13, G2019ZK35
Organisme : the project of improving the research capabilities of young and middle-aged teachers in Guangxi Zhuang Autonomous Region
ID : 2022KY0577
Informations de copyright
© 2024. The Author(s).
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