Versatile and robust genome editing with
Animals
CRISPR-Associated Protein 9
/ chemistry
CRISPR-Cas Systems
Cell Line
Cells, Cultured
Clustered Regularly Interspaced Short Palindromic Repeats
DNA Cleavage
Gene Editing
Humans
Mammals
Mice
Mice, Knockout
Streptococcus thermophilus
/ enzymology
Structure-Activity Relationship
Substrate Specificity
Journal
Genome research
ISSN: 1549-5469
Titre abrégé: Genome Res
Pays: United States
ID NLM: 9518021
Informations de publication
Date de publication:
01 2020
01 2020
Historique:
received:
01
08
2019
accepted:
17
12
2019
pubmed:
5
1
2020
medline:
8
10
2020
entrez:
5
1
2020
Statut:
ppublish
Résumé
Targeting definite genomic locations using CRISPR-Cas systems requires a set of enzymes with unique protospacer adjacent motif (PAM) compatibilities. To expand this repertoire, we engineered nucleases, cytosine base editors, and adenine base editors from the archetypal
Identifiants
pubmed: 31900288
pii: gr.255414.119
doi: 10.1101/gr.255414.119
pmc: PMC6961573
doi:
Substances chimiques
CRISPR-Associated Protein 9
EC 3.1.-
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
107-117Subventions
Organisme : NIGMS NIH HHS
ID : P41 GM103311
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM129325
Pays : United States
Organisme : CIHR
Pays : Canada
Informations de copyright
© 2020 Agudelo et al.; Published by Cold Spring Harbor Laboratory Press.
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