Aryl Hydrocarbon Receptor Modulation by Tuberculosis Drugs Impairs Host Defense and Treatment Outcomes.


Journal

Cell host & microbe
ISSN: 1934-6069
Titre abrégé: Cell Host Microbe
Pays: United States
ID NLM: 101302316

Informations de publication

Date de publication:
12 02 2020
Historique:
received: 08 04 2019
revised: 30 10 2019
accepted: 06 12 2019
pubmed: 7 1 2020
medline: 30 9 2020
entrez: 6 1 2020
Statut: ppublish

Résumé

Antimicrobial resistance in tuberculosis (TB) is a public health threat of global dimension, worsened by increasing drug resistance. Host-directed therapy (HDT) is an emerging concept currently explored as an adjunct therapeutic strategy for TB. One potential host target is the ligand-activated transcription factor aryl hydrocarbon receptor (AhR), which binds TB virulence factors and controls antibacterial responses. Here, we demonstrate that in the context of therapy, the AhR binds several TB drugs, including front line drugs rifampicin (RIF) and rifabutin (RFB), resulting in altered host defense and drug metabolism. AhR sensing of TB drugs modulates host defense mechanisms, notably impairs phagocytosis, and increases TB drug metabolism. Targeting AhR in vivo with a small-molecule inhibitor increases RFB-treatment efficacy. Thus, the AhR markedly impacts TB outcome by affecting both host defense and drug metabolism. As a corollary, we propose the AhR as a potential target for HDT in TB in adjunct to canonical chemotherapy.

Identifiants

pubmed: 31901518
pii: S1931-3128(19)30634-1
doi: 10.1016/j.chom.2019.12.005
pii:
doi:

Substances chimiques

AHR protein, human 0
Antitubercular Agents 0
Basic Helix-Loop-Helix Transcription Factors 0
Receptors, Aryl Hydrocarbon 0
Rifabutin 1W306TDA6S
Rifampin VJT6J7R4TR

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

238-248.e7

Informations de copyright

Copyright © 2019 Elsevier Inc. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of Interests The authors declare no competing interests.

Auteurs

Andreas Puyskens (A)

Department of Immunology, Max Planck Institute for Infection Biology, Charitéplatz 1, Berlin 10117, Germany.

Anne Stinn (A)

Department of Immunology, Max Planck Institute for Infection Biology, Charitéplatz 1, Berlin 10117, Germany; Department for Structural Infection Biology, Center for Structural Systems Biology, Notkestraße 85, Hamburg 22607, Germany.

Michiel van der Vaart (M)

Institute of Biology, Leiden University, Sylviusweg 72, Leiden 2333, the Netherlands.

Annika Kreuchwig (A)

Leibniz-Forschungsinstitut für Molekulare Pharmakologie, Robert-Rössle-Strasse 10, Berlin 13125, Germany.

Jonas Protze (J)

Leibniz-Forschungsinstitut für Molekulare Pharmakologie, Robert-Rössle-Strasse 10, Berlin 13125, Germany.

Gang Pei (G)

Institute of Immunology, Friedrich Loeffler Institute, Südufer 10, Greifswald-Insel Riems 17493, Germany.

Marion Klemm (M)

Department of Immunology, Max Planck Institute for Infection Biology, Charitéplatz 1, Berlin 10117, Germany.

Ute Guhlich-Bornhof (U)

Department of Immunology, Max Planck Institute for Infection Biology, Charitéplatz 1, Berlin 10117, Germany.

Robert Hurwitz (R)

Protein Purification Core Facility, Max Planck Institute for Infection Biology, Charitéplatz 1, Berlin 10117, Germany.

Gopinath Krishnamoorthy (G)

Department of Immunology, Max Planck Institute for Infection Biology, Charitéplatz 1, Berlin 10117, Germany.

Marcel Schaaf (M)

Institute of Biology, Leiden University, Sylviusweg 72, Leiden 2333, the Netherlands.

Gerd Krause (G)

Leibniz-Forschungsinstitut für Molekulare Pharmakologie, Robert-Rössle-Strasse 10, Berlin 13125, Germany.

Annemarie H Meijer (AH)

Institute of Biology, Leiden University, Sylviusweg 72, Leiden 2333, the Netherlands.

Stefan H E Kaufmann (SHE)

Department of Immunology, Max Planck Institute for Infection Biology, Charitéplatz 1, Berlin 10117, Germany; Hagler Institute for Advanced Study at Texas A&M University, College Station, TX 77843, USA. Electronic address: kaufmann@mpiib-berlin.mpg.de.

Pedro Moura-Alves (P)

Department of Immunology, Max Planck Institute for Infection Biology, Charitéplatz 1, Berlin 10117, Germany; Ludwig Institute for Cancer Research, Nuffield Department of Clinical Medicine, University of Oxford, Oxford OX3 7DQ, UK. Electronic address: pedro.mouraalves@ludwig.ox.ac.uk.

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Classifications MeSH