The mechanics of myeloid cells.
cell migration/adhesion
cell motility/contraction
disease
heart/lung/blood vessels
metastasis
Journal
Biology of the cell
ISSN: 1768-322X
Titre abrégé: Biol Cell
Pays: England
ID NLM: 8108529
Informations de publication
Date de publication:
Apr 2020
Apr 2020
Historique:
received:
02
10
2019
revised:
18
12
2019
accepted:
03
01
2020
pubmed:
10
1
2020
medline:
20
11
2020
entrez:
10
1
2020
Statut:
ppublish
Résumé
The effects of cell size, shape and deformability on cellular function have long been a topic of interest. Recently, mechanical phenotyping technologies capable of analysing large numbers of cells in real time have become available. This has important implications for biology and medicine, especially haemato-oncology and immunology, as immune cell mechanical phenotyping, immunologic function, and malignant cell transformation are closely linked and potentially exploitable to develop new diagnostics and therapeutics. In this review, we introduce the technologies used to analyse cellular mechanical properties and review emerging findings following the advent of high throughput deformability cytometry. We largely focus on cells from the myeloid lineage, which are derived from the bone marrow and include macrophages, granulocytes and erythrocytes. We highlight advances in mechanical phenotyping of cells in suspension that are revealing novel signatures of human blood diseases and providing new insights into pathogenesis of these diseases. The contributions of mechanical phenotyping of cells in suspension to our understanding of drug mechanisms, identification of novel therapeutics and monitoring of treatment efficacy particularly in instances of haematologic diseases are reviewed, and we suggest emerging topics of study to explore as high throughput deformability cytometers become prevalent in laboratories across the globe.
Identifiants
pubmed: 31916263
doi: 10.1111/boc.201900084
doi:
Substances chimiques
Antineoplastic Agents
0
Glucocorticoids
0
Types de publication
Journal Article
Review
Langues
eng
Sous-ensembles de citation
IM
Pagination
103-112Subventions
Organisme : GlaxoSmithKline foundation
Organisme : British Heart Foundation
Pays : United Kingdom
Organisme : Bristol-Myers Squibb
Organisme : NIAMS NIH HHS
ID : Intramural Research Program
Pays : United States
Organisme : NHLBI NIH HHS
ID : Intramural Research Program
Pays : United States
Organisme : National Institute for Health Research
Organisme : MedImmune
Organisme : AstraZeneca
Organisme : Medical Research Council
Pays : United Kingdom
Organisme : Wellcome Trust
Pays : United Kingdom
Organisme : NIAMS NIH HHS
ID : Intramural Research Program
Pays : United States
Organisme : NHLBI NIH HHS
ID : Intramural Research Program
Pays : United States
Informations de copyright
© 2020 Société Française des Microscopies and Société de Biologie Cellulaire de France. Published by John Wiley & Sons Ltd.
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