Non-essentiality of canonical cell division genes in the planctomycete Planctopirus limnophila.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
09 01 2020
09 01 2020
Historique:
received:
11
04
2019
accepted:
19
12
2019
entrez:
11
1
2020
pubmed:
11
1
2020
medline:
11
11
2020
Statut:
epublish
Résumé
Most bacteria divide by binary fission using an FtsZ-based mechanism that relies on a multi-protein complex, the divisome. In the majority of non-spherical bacteria another multi-protein complex, the elongasome, is also required for the maintenance of cell shape. Components of these multi-protein assemblies are conserved and essential in most bacteria. Here, we provide evidence that at least three proteins of these two complexes are not essential in the FtsZ-less ovoid planctomycete bacterium Planctopirus limnophila which divides by budding. We attempted to construct P. limnophila knock-out mutants of the genes coding for the divisome proteins FtsI, FtsK, FtsW and the elongasome protein MreB. Surprisingly, ftsI, ftsW and mreB could be deleted without affecting the growth rate. On the other hand, the conserved ftsK appeared to be essential in this bacterium. In conclusion, the canonical bacterial cell division machinery is not essential in P. limnophila and this bacterium divides via budding using an unknown mechanism.
Identifiants
pubmed: 31919386
doi: 10.1038/s41598-019-56978-8
pii: 10.1038/s41598-019-56978-8
pmc: PMC6952346
doi:
Substances chimiques
Actins
0
Bacterial Proteins
0
Penicillin-Binding Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
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