Up-Regulated MicroRNA-27b Promotes Adipocyte Differentiation via Induction of Acyl-CoA Thioesterase 2 Expression.
3T3-L1 Cells
Adipocytes
/ pathology
Adipogenesis
/ genetics
Animals
Cell Differentiation
/ genetics
Cell Line
Mice
MicroRNAs
/ genetics
Non-alcoholic Fatty Liver Disease
/ genetics
Palmitoyl-CoA Hydrolase
/ genetics
RNA Interference
/ physiology
Transcriptional Activation
/ genetics
Up-Regulation
/ genetics
Journal
BioMed research international
ISSN: 2314-6141
Titre abrégé: Biomed Res Int
Pays: United States
ID NLM: 101600173
Informations de publication
Date de publication:
2019
2019
Historique:
received:
22
04
2019
revised:
22
07
2019
accepted:
04
08
2019
entrez:
14
1
2020
pubmed:
14
1
2020
medline:
11
6
2020
Statut:
epublish
Résumé
Nonalcoholic fatty liver disease (NAFLD) is characterized by a spectrum of liver pathologies, from simple steatosis to steatohepatitis. Recent studies have increasingly noted the aberrant expression of microRNAs closely related to NAFLD pathologies. We have previously shown the presence of increased levels of microRNA-27b (miR-27b) in patients with NAFLD. In this study, we investigated the role of miR-27b in NAFLD by examining the impact of up-regulated miR-27b on the differentiation of preadipocytes into mature adipocytes. We found that miR-27b-3p remarkably enhances the adipocyte differentiation of 3T3-L1 cells associated with lipid accumulation and intracellular triglyceride contents. Furthermore, we have demonstrated not only that miR-27b-3p induces acyl-CoA thioesterase 2 (ACOT2) expression in 3T3-L1 cells, but also that the knockdown of ACOT2 suppresses lipid accumulation and adipocyte differentiation in both the presence and absence of miR-27b-3p treatment. Our data strongly suggest that the miR-27b-ACOT2 axis is an important pathway in adipocyte differentiation and may play a role in the pathogenesis of NAFLD.
Identifiants
pubmed: 31930115
doi: 10.1155/2019/2916243
pmc: PMC6942750
doi:
Substances chimiques
MicroRNAs
0
Mirn27 microRNA, mouse
0
Palmitoyl-CoA Hydrolase
EC 3.1.2.2
mitochondrial acyl-CoA thioesterase I, mouse
EC 3.1.2.2
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
2916243Informations de copyright
Copyright © 2019 Yuka Murata et al.
Déclaration de conflit d'intérêts
The authors declare that they have no conflicts of interest.
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