Optogenetics: Rho GTPases Activated by Light in Living Macrophages.
LOV2
Optogenetics
Photoactivatable proteins
Rho GTPases
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2020
2020
Historique:
entrez:
16
1
2020
pubmed:
16
1
2020
medline:
28
1
2021
Statut:
ppublish
Résumé
Genetically encoded optogenetic tools are increasingly popular and useful for perturbing signaling pathways with high spatial and temporal resolution in living cells. Here, we show basic procedures employed to implement optogenetics of Rho GTPases in a macrophage cell line. Methods described here are generally applicable to other genetically encoded optogenetic tools utilizing the blue-green spectrum of light for activation, designed for specific proteins and enzymatic targets important for immune cell functions.
Identifiants
pubmed: 31939189
doi: 10.1007/978-1-0716-0247-8_24
pmc: PMC7032845
mid: NIHMS1069269
doi:
Substances chimiques
DNA-Binding Proteins
0
rho GTP-Binding Proteins
EC 3.6.5.2
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
281-293Subventions
Organisme : NCI NIH HHS
ID : P30 CA013330
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM129098
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM132098
Pays : United States
Organisme : NIGMS NIH HHS
ID : T32 GM007491
Pays : United States
Références
Mol Plant. 2012 May;5(3):533-44
pubmed: 22431563
Nature. 2009 Sep 3;461(7260):104-8
pubmed: 19693014
Methods Mol Biol. 2013;1046:359-70
pubmed: 23868600
J Immunol. 2016 Apr 15;196(8):3479-93
pubmed: 26951800
Methods Mol Biol. 2017;1519:125-143
pubmed: 27815877
PLoS One. 2014 May 05;9(5):e96469
pubmed: 24798463
Curr Biol. 2011 Apr 26;21(8):635-44
pubmed: 21474314
Nat Methods. 2016 Sep;13(9):755-8
pubmed: 27427858
Annu Rev Plant Biol. 2012;63:49-72
pubmed: 22136567
Science. 2016 Dec 16;354(6318):1441-1444
pubmed: 27980211
Chem Rev. 2017 May 10;117(9):6423-6446
pubmed: 28401765
Sci Rep. 2017 Aug 17;7(1):8547
pubmed: 28819224
Nat Chem Biol. 2018 Jun;14(6):591-600
pubmed: 29686359
Methods Mol Biol. 2012;827:215-34
pubmed: 22144278
Annu Rev Biochem. 2015;84:519-50
pubmed: 25706899
J Cell Biol. 2017 Dec 4;216(12):4331-4349
pubmed: 29061650
J Exp Med. 1997 Nov 3;186(9):1487-94
pubmed: 9348306
Nat Cell Biol. 2014 Jun;16(6):574-86
pubmed: 24859002
Methods Cell Biol. 2013;114:593-609
pubmed: 23931524
Front Mol Biosci. 2015 May 12;2:18
pubmed: 25988185
Methods Enzymol. 2011;497:393-407
pubmed: 21601095
Curr Protoc Cell Biol. 2016 Dec 1;73:21.10.1-21.10.14
pubmed: 27906450
Trends Biotechnol. 2015 Feb;33(2):92-100
pubmed: 25529484