Identification of 56 Proteins Involved in Embryo-Maternal Interactions in the Bovine Oviduct.
4–6 cell
bovine
cattle
embryo
fallopian tube
morula
oviduct
proteomics
secretions
tubal fluid
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
11 Jan 2020
11 Jan 2020
Historique:
received:
06
12
2019
revised:
08
01
2020
accepted:
10
01
2020
entrez:
17
1
2020
pubmed:
17
1
2020
medline:
4
6
2020
Statut:
epublish
Résumé
The bovine embryo develops in contact with the oviductal fluid (OF) during the first 4-5 days of pregnancy. The aim of this study was to decipher the protein interactions occurring between the developing embryo and surrounding OF. In-vitro produced 4-6 cell and morula embryos were incubated or not (controls) in post-ovulatory OF (OF-treated embryos) and proteins were then analyzed and quantified by high resolution mass spectrometry (MS) in both embryo groups and in OF. A comparative analysis of MS data allowed the identification and quantification of 56 embryo-interacting proteins originated from the OF, including oviductin (OVGP1) and several annexins (ANXA1, ANXA2, ANXA4) as the most abundant ones. Some embryo-interacting proteins were developmental stage-specific, showing a modulating role of the embryo in protein interactions. Three interacting proteins (OVGP1, ANXA1 and PYGL) were immunolocalized in the perivitelline space and in blastomeres, showing that OF proteins were able to cross the zona pellucida and be taken up by the embryo. Interacting proteins were involved in a wide range of functions, among which metabolism and cellular processes were predominant. This study identified for the first time a high number of oviductal embryo-interacting proteins, paving the way for further targeted studies of proteins potentially involved in the establishment of pregnancy in cattle.
Identifiants
pubmed: 31940782
pii: ijms21020466
doi: 10.3390/ijms21020466
pmc: PMC7013689
pii:
doi:
Substances chimiques
Annexins
0
Proteome
0
Serine Endopeptidases
EC 3.4.21.-
oviductin
EC 3.4.21.-
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Evolution
ID : No grant number
Organisme : Association Nationale de la Recherche et de la Technologie
ID : 2017/0684
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