Distinct MCM10 Proteasomal Degradation Profiles by Primate Lentiviruses Vpr Proteins.
Cell Cycle Checkpoints
DNA Damage
Gene Products, vpr
/ genetics
HEK293 Cells
HIV-1
/ genetics
HeLa Cells
Humans
Lentiviruses, Primate
/ chemistry
Minichromosome Maintenance Proteins
/ genetics
Phylogeny
Proteasome Endopeptidase Complex
/ metabolism
Proteolysis
Simian Immunodeficiency Virus
/ genetics
DNA damage response
G2/M arrest
MCM10
Vpr
primate lentiviruses
proteasomal degradation
Journal
Viruses
ISSN: 1999-4915
Titre abrégé: Viruses
Pays: Switzerland
ID NLM: 101509722
Informations de publication
Date de publication:
15 01 2020
15 01 2020
Historique:
received:
11
11
2019
revised:
28
12
2019
accepted:
10
01
2020
entrez:
19
1
2020
pubmed:
19
1
2020
medline:
18
2
2021
Statut:
epublish
Résumé
Viral protein R (Vpr) is an accessory protein found in various primate lentiviruses, including human immunodeficiency viruses type 1 and 2 (HIV-1 and HIV-2) as well as simian immunodeficiency viruses (SIVs). Vpr modulates many processes during viral lifecycle via interaction with several of cellular targets. Previous studies showed that HIV-1 Vpr strengthened degradation of Mini-chromosome Maintenance Protein10 (MCM10) by manipulating DCAF1-Cul4-E3 ligase in proteasome-dependent pathway. However, whether Vpr from other primate lentiviruses are also associated with MCM10 degradation and the ensuing impact remain unknown. Based on phylogenetic analyses, a panel of primate lentiviruses Vpr/x covering main virus lineages was prepared. Distinct MCM10 degradation profiles were mapped and HIV-1, SIVmus and SIVrcm Vprs induced MCM10 degradation in proteasome-dependent pathway. Colocalization and interaction between MCM10 with these Vprs were also observed. Moreover, MCM10 2-7 interaction region was identified as a determinant region susceptible to degradation. However, MCM10 degradation did not alleviate DNA damage response induced by these Vpr proteins. MCM10 degradation by HIV-1 Vpr proteins was correlated with G
Identifiants
pubmed: 31952107
pii: v12010098
doi: 10.3390/v12010098
pmc: PMC7019430
pii:
doi:
Substances chimiques
Gene Products, vpr
0
Proteasome Endopeptidase Complex
EC 3.4.25.1
Minichromosome Maintenance Proteins
EC 3.6.4.12
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
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