Human monocytes subjected to ischaemia/reperfusion inhibit angiogenesis and wound healing in vitro.
cytokines
ischaemia/reperfusion injury
monocytes
neoangiogenesis
remodelling
wound healing
Journal
Cell proliferation
ISSN: 1365-2184
Titre abrégé: Cell Prolif
Pays: England
ID NLM: 9105195
Informations de publication
Date de publication:
Feb 2020
Feb 2020
Historique:
received:
23
07
2019
revised:
15
11
2019
accepted:
13
12
2019
pubmed:
21
1
2020
medline:
7
3
2020
entrez:
21
1
2020
Statut:
ppublish
Résumé
The sequence of initial tissue ischaemia and consecutive blood flow restoration leads to ischaemia/reperfusion (I/R) injury, which is typically characterized by a specific inflammatory response. Migrating monocytes seem to mediate the immune response in ischaemic tissues and influence detrimental as well as regenerative effects during I/R injury. To clarify the role of classical monocytes in I/R injury, isolated human monocytes were subjected to I/R in vitro (3 hours ischaemia followed by 24 hours of reperfusion). Cellular resilience, monocyte differentiation, cytokine secretion, as well as influence on endothelial tube formation, migration and cell recovery were investigated. We show that I/R supported an enhanced resilience of monocytes and induced intracellular phosphorylation of the prosurvival molecules Erk1/2 and Akt. FACS analysis showed no major alteration in monocyte subtype differentiation and surface marker expression under I/R. Further, our experiments revealed that I/R changes the cytokine secretion pattern, release of angiogenesis associated proteins and MMP-9 activity in supernatants of monocytes exposed to I/R. Supernatants from monocytes subjected to I/R attenuated endothelial tube formation as indicator for angiogenesis as well as endothelial cell migration and recovery. In summary, monocytes showed no significant change in cellular integrity and monocyte subtype after I/R. Functionally, monocytes might have a rather detrimental influence during the initial phase of I/R, suppressing endothelial cell migration and neoangiogenesis.
Identifiants
pubmed: 31957193
doi: 10.1111/cpr.12753
pmc: PMC7048205
doi:
Substances chimiques
Cytokines
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e12753Subventions
Organisme : Schleswig-Holstein's Ministry of Science, Economy and Transport
ID : F359921
Informations de copyright
© 2020 The Authors. Cell Proliferation Published by John Wiley & Sons Ltd.
Références
J Am Coll Cardiol. 2014 Apr 22;63(15):1556-66
pubmed: 24361318
PLoS One. 2009 Oct 26;4(10):e7588
pubmed: 19855846
Am J Respir Cell Mol Biol. 2002 Feb;26(2):224-30
pubmed: 11804874
IUBMB Life. 2015 Mar;67(3):145-59
pubmed: 25899846
Oncotarget. 2016 Jan 26;7(4):4310-25
pubmed: 26713602
Biosci Rep. 2017 Sep 19;37(5):
pubmed: 28842516
Immunity. 2013 Mar 21;38(3):555-69
pubmed: 23477737
Front Immunol. 2014 Nov 28;5:614
pubmed: 25506346
J Biomed Sci. 2013 Dec 23;20:100
pubmed: 24364919
Mol Cell Biol. 2010 Oct;30(20):4901-21
pubmed: 20696840
Int J Cardiol. 2008 Nov 12;130(2):147-58
pubmed: 18656272
Am J Pathol. 2015 Oct;185(10):2596-606
pubmed: 26118749
J Wound Care. 2017 Jul 2;26(7):381-397
pubmed: 28704150
J Immunol. 1999 May 15;162(10):6217-25
pubmed: 10229867
Pharmacol Ther. 2018 Jun;186:73-87
pubmed: 29330085
J Am Coll Cardiol. 2017 Jul 4;70(1):1-25
pubmed: 28527533
Ann Plast Surg. 2014 Feb;72(2):253-60
pubmed: 23241775
Pharmacol Res. 2008 Aug;58(2):88-111
pubmed: 18620057
Nat Med. 2011 Nov 07;17(11):1391-401
pubmed: 22064429
Microcirculation. 2014 Feb;21(2):148-58
pubmed: 24125396
Antioxid Redox Signal. 2007 Nov;9(11):1979-89
pubmed: 17949278
J Immunol. 2009 Jun 15;182(12):7974-81
pubmed: 19494322
J Cell Physiol. 2016 Dec;231(12):2599-621
pubmed: 27187048
Prog Mol Biol Transl Sci. 2017;147:1-73
pubmed: 28413025
Cardiovasc Res. 2010 Mar 1;85(4):649-60
pubmed: 19805399
Cell Physiol Biochem. 2018;46(4):1650-1667
pubmed: 29694958
Stem Cells. 2006 Dec;24(12):2733-43
pubmed: 16888284
Exp Cell Res. 2017 Apr 15;353(2):109-114
pubmed: 28300560
Dis Model Mech. 2013 Nov;6(6):1507-14
pubmed: 24046359
Lancet. 2014 May 31;383(9932):1933-43
pubmed: 24831770
Eur J Pharmacol. 2010 Oct 25;645(1-3):39-46
pubmed: 20655305
J Cereb Blood Flow Metab. 2001 Oct;21(10):1223-31
pubmed: 11598500
Vascular. 2012 Aug;20(4):210-6
pubmed: 22896663
World J Cardiol. 2010 Oct 26;2(10):325-32
pubmed: 21160610
Circulation. 2010 Jun 8;121(22):2437-45
pubmed: 20530020
Eur J Anaesthesiol. 2018 Mar;35(3):200-207
pubmed: 28937529
Eur J Immunol. 2012 Jul;42(7):1804-14
pubmed: 22585447
Mol Cell Biol. 1993 Oct;13(10):6231-40
pubmed: 8413223
PLoS One. 2012;7(6):e39381
pubmed: 22761777
FEBS J. 2016 Feb;283(3):413-24
pubmed: 26513405
Biomed Pharmacother. 2016 Dec;84:1078-1087
pubmed: 27780136
Mol Cell Biol. 1990 Mar;10(3):1281-6
pubmed: 2406568
J Immunol Methods. 2004 May;288(1-2):123-34
pubmed: 15183091
Mol Cell Biol. 1993 Oct;13(10):6137-46
pubmed: 8413215
Nat Protoc. 2007;2(3):481-5
pubmed: 17406610
Neurobiol Dis. 2007 Jan;25(1):54-64
pubmed: 17011206
Exp Cell Res. 2014 Mar 10;322(1):62-70
pubmed: 24394542
Mol Cell Biol. 1990 Apr;10(4):1498-506
pubmed: 2181276
Plast Reconstr Surg. 2016 Mar;137(3):545e-553e
pubmed: 26910699
J Cell Mol Med. 2010 Mar;14(3):553-63
pubmed: 19754667
Cell Prolif. 2020 Feb;53(2):e12753
pubmed: 31957193
Cancer Res. 2014 Mar 1;74(5):1287-93
pubmed: 24556724
J Immunol. 2007 Jun 1;178(11):7097-109
pubmed: 17513759
Macromol Biosci. 2012 Jul;12(7):901-10
pubmed: 22696505
Int Rev Cell Mol Biol. 2012;298:229-317
pubmed: 22878108
J Leukoc Biol. 2014 Feb;95(2):233-41
pubmed: 24168857