CD248 and integrin alpha-8 are candidate markers for differentiating lung fibroblast subtypes.
Aged
Animals
Antigens, CD
/ metabolism
Antigens, Ly
/ metabolism
Antigens, Neoplasm
/ metabolism
Connective Tissue
/ pathology
Connective Tissue Cells
Elastic Tissue
Fibroblasts
/ classification
Flow Cytometry
Humans
Idiopathic Pulmonary Fibrosis
/ pathology
In Vitro Techniques
Integrin alpha Chains
/ metabolism
Lung
/ cytology
Male
Membrane Proteins
/ metabolism
Mice
Middle Aged
Tissue Array Analysis
Collagen fibers
Elastic fibers
Fibroblast
Fibrosis
Idiopathic pulmonary fibrosis
Lung
Journal
BMC pulmonary medicine
ISSN: 1471-2466
Titre abrégé: BMC Pulm Med
Pays: England
ID NLM: 100968563
Informations de publication
Date de publication:
21 Jan 2020
21 Jan 2020
Historique:
received:
03
05
2019
accepted:
13
01
2020
entrez:
23
1
2020
pubmed:
23
1
2020
medline:
20
11
2020
Statut:
epublish
Résumé
Lung fibrosis is a serious life-threatening condition whose manifestation varies according to the localization and characteristics of fibroblasts, which are considered heterogeneous. Therefore, to better understand the pathology and improve diagnosis and treatment of this disease, it is necessary to elucidate the nature of this heterogeneity and identify markers for the accurate classification of human lung fibroblast subtypes. We characterized distinct mouse lung fibroblast subpopulations isolated by fluorescence-activated cell sorting (FACS) and performed microarray analysis to identify molecular markers that could be useful for human lung fibroblast classification. Based on the expression of these markers, we evaluated the fibroblast-like cell subtype localization in normal human lung samples and lung samples from patients with idiopathic pulmonary fibrosis (IPF). Mouse lung fibroblasts were classified into Sca-1 CD248
Sections du résumé
BACKGROUND
BACKGROUND
Lung fibrosis is a serious life-threatening condition whose manifestation varies according to the localization and characteristics of fibroblasts, which are considered heterogeneous. Therefore, to better understand the pathology and improve diagnosis and treatment of this disease, it is necessary to elucidate the nature of this heterogeneity and identify markers for the accurate classification of human lung fibroblast subtypes.
METHODS
METHODS
We characterized distinct mouse lung fibroblast subpopulations isolated by fluorescence-activated cell sorting (FACS) and performed microarray analysis to identify molecular markers that could be useful for human lung fibroblast classification. Based on the expression of these markers, we evaluated the fibroblast-like cell subtype localization in normal human lung samples and lung samples from patients with idiopathic pulmonary fibrosis (IPF).
RESULTS
RESULTS
Mouse lung fibroblasts were classified into Sca-1
CONCLUSION
CONCLUSIONS
CD248
Identifiants
pubmed: 31964365
doi: 10.1186/s12890-020-1054-9
pii: 10.1186/s12890-020-1054-9
pmc: PMC6975017
doi:
Substances chimiques
Antigens, CD
0
Antigens, Ly
0
Antigens, Neoplasm
0
CD248 protein, human
0
CD248 protein, mouse
0
ITGA8 protein, human
0
Integrin alpha Chains
0
Ly6a protein, mouse
0
Membrane Proteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
21Subventions
Organisme : Japan Society for the Promotion of Science
ID : 16K08736
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