Morphometric, subcellular, in vitro fertilisation and embryonic developmental assessment of mouse oocytes produced by anti-inhibin serum or pregnant mare serum gonadotrophin superovulation.
Animals
Blastocyst
/ physiology
Chorionic Gonadotropin
/ pharmacology
Embryo Culture Techniques
Female
Fertility Agents, Female
/ pharmacology
Fertilization in Vitro
Gonadotropins, Equine
/ pharmacology
Immune Sera
/ pharmacology
Inhibins
/ antagonists & inhibitors
Mice, Inbred C57BL
Mice, Inbred ICR
Oocyte Retrieval
Oocytes
/ drug effects
Ovulation
/ drug effects
Pregnancy
Superovulation
Journal
Reproduction, fertility, and development
ISSN: 1031-3613
Titre abrégé: Reprod Fertil Dev
Pays: Australia
ID NLM: 8907465
Informations de publication
Date de publication:
Mar 2020
Mar 2020
Historique:
received:
07
04
2019
accepted:
01
08
2019
pubmed:
24
1
2020
medline:
13
1
2021
entrez:
24
1
2020
Statut:
ppublish
Résumé
This study compared the morphometric, subcellular characteristics, in vitro fertilisation (IVF) and embryonic developmental potential of metaphase II (MII) mouse oocytes obtained from females superovulated with either anti-inhibin serum-human chorionic gonadotrophin (AIS-hCG) or pregnant mare serum gonadotrophin (PMSG)-hCG. The oocyte's quantity, quality, zona pellucida (ZP) thickness, perivitelline space (PVS), diameter, microtubules, F-actin, cortical granules (CGs) and mitochondrial distribution were determined. Superovulation using AIS-hCG resulted in a higher numbers of oocyte/donor compared with PMSG-hCG (P=0.002). There was no difference in morphologically normal and abnormal oocytes between AIS-hCG and PMSG-hCG (P=0.425 and P=0.194, respectively). The morphometric measurements showed no difference in oocyte diameter between AIS-hCG and PMSG-hCG (P=0.289). However, the thickness of the ZP of oocytes from AIS-hCG females was decreased compared with PMSG-hCG (P<0.001). The PVS of oocytes from the AIS-hCG was larger than with PMSG-hCG (P<0.001). The microtubules of oocytes from both AIS-hCG and PMSG-hCG were normal, although there was an increased fluorescence intensity in the AIS-hCG oocytes (P<0.001). The F-actin and CGs distribution in oocytes from both AIS-hCG and PMSG-hCG were similar (P=0.330 and P=0.13, respectively). Although the oocytes from PMSG-hCG females had homogenously distributed mitochondria, AIS-hCG oocytes showed more peripheral distribution with no differences in fluorescence intensity (P=0.137). The blastocyst development rates after IVF with fresh sperm showed no difference between AIS-hCG and PMSG-hCG (P=0.235). These data suggested that AIS-hCG superovulation produces high numbers of morphologically normal oocytes that also possess normal subcellular structures, good morphological characteristics and had high invitro embryonic developmental potential.
Identifiants
pubmed: 31972126
pii: RD19131
doi: 10.1071/RD19131
pmc: PMC8020730
mid: NIHMS1684503
doi:
Substances chimiques
Chorionic Gonadotropin
0
Fertility Agents, Female
0
Gonadotropins, Equine
0
Immune Sera
0
Inhibins
57285-09-3
Types de publication
Comparative Study
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
474-483Subventions
Organisme : NIH HHS
ID : U42 OD010918
Pays : United States
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