Impact of PTEN/SOCS3 deletion on amelioration of dendritic shrinkage of retinal ganglion cells after optic nerve injury.
Animals
Dendrites
/ physiology
Dependovirus
/ genetics
Female
Gene Deletion
Genetic Vectors
Male
Mice
Mice, Inbred C57BL
Mice, Knockout
Mice, Transgenic
Microscopy, Confocal
Nerve Crush
Nerve Fibers
/ physiology
Nerve Regeneration
/ physiology
Optic Nerve Injuries
/ physiopathology
PTEN Phosphohydrolase
/ genetics
Retinal Ganglion Cells
/ physiology
Suppressor of Cytokine Signaling 3 Protein
/ genetics
Dendritic shrinkage
Optic nerve injury
Phosphatase and tensin homolog (PTEN)
Retinal ganglion cell
Suppressor of cytokine signaling 3 (SOCS3)
Journal
Experimental eye research
ISSN: 1096-0007
Titre abrégé: Exp Eye Res
Pays: England
ID NLM: 0370707
Informations de publication
Date de publication:
03 2020
03 2020
Historique:
received:
17
04
2019
revised:
02
01
2020
accepted:
16
01
2020
pubmed:
24
1
2020
medline:
24
10
2020
entrez:
24
1
2020
Statut:
ppublish
Résumé
Retinal ganglion cell (RGC) degeneration, leading to irreversible blindness in chronic optic neuropathies, commonly begins with dendritic shrinkage followed by axon degeneration. Although limited axon regeneration in the optic nerve is possible with a genetic deletion of PTEN/SOCS3 after optic nerve injury, the roles of PTEN/SOCS3 on dendritic preservation and regeneration remain unclear. This study investigated the effect of PTEN/SOCS3 genetic deletion on the structural integrity of RGC dendrites and axons in the retina following optic nerve crush. Using time-lapse, in vivo confocal scanning laser ophthalmoscopy to serially image dendritic and axonal arborizations of RGCs over six months after injury, RGC dendrites and axons were only preserved in Thy-1-YFP/PTEN
Identifiants
pubmed: 31972211
pii: S0014-4835(19)30203-9
doi: 10.1016/j.exer.2020.107938
pii:
doi:
Substances chimiques
Socs3 protein, mouse
0
Suppressor of Cytokine Signaling 3 Protein
0
PTEN Phosphohydrolase
EC 3.1.3.67
Pten protein, mouse
EC 3.1.3.67
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
107938Informations de copyright
Copyright © 2020. Published by Elsevier Ltd.
Déclaration de conflit d'intérêts
Declaration of competing interest The authors declare that they have no competing interests.