Molecular epidemiology of Enterocytozoon bieneusi and Encephalitozoon sp., among immunocompromised and immunocompetent subjects in Iran.
Adolescent
Adult
Aged
Child
Child, Preschool
DNA, Ribosomal Spacer
Encephalitozoon
/ classification
Enterocytozoon
/ classification
Female
Genes, Fungal
Humans
Immunocompromised Host
Iran
/ epidemiology
Male
Microsporidiosis
/ epidemiology
Middle Aged
Molecular Epidemiology
Opportunistic Infections
/ epidemiology
Pathology, Molecular
/ methods
Phylogeny
RNA, Ribosomal, 18S
Young Adult
Encephalitozoon sp.
Enterocytozoon bieneusi
Healthy subjects
Immunodeficiency
Iran
Molecular analysis
Journal
Microbial pathogenesis
ISSN: 1096-1208
Titre abrégé: Microb Pathog
Pays: England
ID NLM: 8606191
Informations de publication
Date de publication:
Apr 2020
Apr 2020
Historique:
received:
25
09
2019
revised:
18
01
2020
accepted:
19
01
2020
pubmed:
24
1
2020
medline:
2
2
2021
entrez:
24
1
2020
Statut:
ppublish
Résumé
Intestinal microsporidiosis is known as an opportunistic infection in immunocompromised patients. The current study aimed to investigate intestinal microsporidia infection in human subjects with/without immunodeficiency. Totally, 600 stool samples were collected from immunocompromised (254) and immunocompetent (346) subjects. DNA extraction was performed and the SSU rRNA and the ITS genes were amplified to detect and characterize microsporidia and the relevant genotypes. Phylogenetic trees were drawn using MEGA7 software to illustrate the correlation between isolates. From 600 enrolled subjects, 283 and 317 were male and female, respectively. The average age ± SD of all tested subjects was 28.85 ± 26.92. The results of PCR demonstrated the presence of E. bieneusi and Encephalitozoon sp., among 10/600 (1.67%) and 26/600 (4.33%) of samples, respectively. Accordingly, E. bieneusi was seen among 4/346 (1.15%), 1/53 (1.88%), 3/124 (2.42%), and 2/63 (3.17%), and Encephalitozoon sp., was detected from 17/346 (4.91%), 3/53 (5.36%), 4/124 (3.22%) and 2/63 (3.17%) of healthy subjects, RA patients, cancer patients, and transplantation recipients, respectively. Statistical significant correlation was not seen between the presence of microsporidia and age, gender, stool appearance, and geographical region. Molecular analysis showed that all E. bieneusi were the genotype D. Phylogenetic tree demonstrated no classification according to the presence/absence of immunodeficiency, geographical locations and presence of diarrhea. The high prevalence of Encephalitozoon sp., in comparison to E. bieneusi in this study suggested the importance of this genus alongside with E. bieneusi in Iran. In addition, predominance of the genotype D highlighted the wide distribution of this genotype in Iran.
Identifiants
pubmed: 31972268
pii: S0882-4010(19)31713-9
doi: 10.1016/j.micpath.2020.103988
pii:
doi:
Substances chimiques
DNA, Ribosomal Spacer
0
RNA, Ribosomal, 18S
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
103988Informations de copyright
Copyright © 2020 Elsevier Ltd. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of competing interest The authors declare that they have no conflict of interest.