Optimization of sortase A ligation for flexible engineering of complex protein systems.
GTPase-activating protein (GAP)
SrtA
cyclic peptide
cyclic proteins
intrinsically disordered region
lipidated proteins
nanodisc
post-translational modification (PTM)
protein engineering
protein ligation
protein tagging
segmental labeling
sortase
sortase A
structural biology
Journal
The Journal of biological chemistry
ISSN: 1083-351X
Titre abrégé: J Biol Chem
Pays: United States
ID NLM: 2985121R
Informations de publication
Date de publication:
28 02 2020
28 02 2020
Historique:
received:
22
11
2019
revised:
22
01
2020
pubmed:
25
1
2020
medline:
22
10
2020
entrez:
25
1
2020
Statut:
ppublish
Résumé
Engineering and bioconjugation of proteins is a critically valuable tool that can facilitate a wide range of biophysical and structural studies. The ability to orthogonally tag or label a domain within a multidomain protein may be complicated by undesirable side reactions to noninvolved domains. Furthermore, the advantages of segmental (or domain-specific) isotopic labeling for NMR, or deuteration for neutron scattering or diffraction, can be realized by an efficient ligation procedure. Common methods-expressed protein ligation, protein trans-splicing, and native chemical ligation-each have specific limitations. Here, we evaluated the use of different variants of
Identifiants
pubmed: 31974162
pii: S0021-9258(17)49004-1
doi: 10.1074/jbc.RA119.012039
pmc: PMC7049969
pii:
doi:
Substances chimiques
Bacterial Proteins
0
Aminoacyltransferases
EC 2.3.2.-
sortase A
EC 2.3.2.-
Cysteine Endopeptidases
EC 3.4.22.-
Types de publication
Journal Article
Research Support, N.I.H., Intramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
2664-2675Subventions
Organisme : Intramural NIH HHS
ID : ZIA BC011131
Pays : United States
Organisme : Intramural NIH HHS
ID : ZIA BC011132
Pays : United States
Organisme : Intramural NIH HHS
ID : ZIA BC011419
Pays : United States
Références
Nat Methods. 2017 Jan;14(1):49-52
pubmed: 27869813
Curr Protoc Protein Sci. 2017 Aug 1;89:15.3.1-15.3.19
pubmed: 28762490
Biotechnol J. 2015 Sep;10(9):1487-92
pubmed: 25864513
Proc Natl Acad Sci U S A. 2011 Jul 12;108(28):11399-404
pubmed: 21697512
J Am Chem Soc. 2013 Feb 6;135(5):1919-25
pubmed: 23294159
Chembiochem. 2018 Sep 17;19(18):1927-1933
pubmed: 29947468
Anal Sci. 2019 Jan 10;35(1):5-27
pubmed: 30318491
Chem Commun (Camb). 2015 Aug 14;51(63):12548-51
pubmed: 26152789
Methods Enzymol. 2015;565:389-422
pubmed: 26577740
FASEB J. 2011 Aug;25(8):2650-8
pubmed: 21525488
J Biol Chem. 2007 Aug 10;282(32):23129-39
pubmed: 17545669
Nat Rev Mol Cell Biol. 2011 Jun;12(6):362-75
pubmed: 21587297
Nat Commun. 2019 Jun 24;10(1):2775
pubmed: 31235796
J Biomol NMR. 2011 Jan;49(1):3-7
pubmed: 21188472
Annu Rev Biophys Biomol Struct. 2006;35:225-49
pubmed: 16689635
J Am Chem Soc. 2019 Jul 31;141(30):11881-11891
pubmed: 31293161
Chem Biol Drug Des. 2013 Oct;82(4):418-28
pubmed: 23701677
Proc Natl Acad Sci U S A. 1998 Aug 4;95(16):9226-31
pubmed: 9689062
Chem Biol. 2008 Feb;15(2):128-36
pubmed: 18291317
J Biol Chem. 2009 Sep 4;284(36):24465-77
pubmed: 19592495
J Am Chem Soc. 2017 Apr 19;139(15):5351-5358
pubmed: 28199119
Nat Protoc. 2006;1(2):749-54
pubmed: 17406304
EMBO J. 2013 Sep 11;32(18):2504-16
pubmed: 23942235
Annu Rev Cell Dev Biol. 2007;23:579-611
pubmed: 17506703
Proc Natl Acad Sci U S A. 1998 Jun 9;95(12):6705-10
pubmed: 9618476
Chembiochem. 2018 Jan 18;19(2):185-195
pubmed: 29124839
Nat Protoc. 2015 May;10(5):780-91
pubmed: 25906116
Nature. 2017 Oct 4;550(7674):53-60
pubmed: 28980641
Structure. 2017 May 2;25(5):794-805.e5
pubmed: 28434917
J Am Chem Soc. 2012 Jan 18;134(2):800-3
pubmed: 22175226
Nat Chem Biol. 2006 Nov;2(11):584-90
pubmed: 17051234
Nat Chem Biol. 2007 Nov;3(11):707-8
pubmed: 17891153
Curr Opin Chem Biol. 2014 Oct;22:122-8
pubmed: 25299574
FEBS Lett. 1999 Aug 6;456(2):295-300
pubmed: 10456327
PLoS One. 2016 Apr 29;11(4):e0154607
pubmed: 27128490
Mol Cell Biol. 1998 Dec;18(12):7038-51
pubmed: 9819391
J Biomol NMR. 2015 Sep;63(1):1-8
pubmed: 26319988
Biotechnol Bioeng. 2012 Dec;109(12):2955-61
pubmed: 22729808
J Biol Chem. 1995 Jun 16;270(24):14809-15
pubmed: 7782347
Curr Opin Struct Biol. 2016 Jun;38:111-8
pubmed: 27318815
J Biomol NMR. 2018 Aug;71(4):225-235
pubmed: 29536230
Nat Struct Mol Biol. 2010 Jul;17(7):876-81
pubmed: 20601958
Science. 1994 Nov 4;266(5186):776-9
pubmed: 7973629
Mol Cell. 2009 Jun 26;34(6):674-85
pubmed: 19560420
J Am Chem Soc. 2008 Dec 3;130(48):16338-43
pubmed: 18989959
Structure. 2015 Nov 3;23(11):1977-88
pubmed: 26365802
Proc Natl Acad Sci U S A. 2001 Dec 4;98(25):14422-7
pubmed: 11724934
Sci Rep. 2016 Aug 18;6:31899
pubmed: 27534437
J Mol Biol. 2018 Aug 3;430(16):2275-2277
pubmed: 29906412
Bioconjug Chem. 2016 Aug 17;27(8):1771-83
pubmed: 27444727
Biochemistry. 2019 Mar 12;58(10):1423-1431
pubmed: 30735034
Chemistry. 2018 Apr 11;24(21):5493-5499
pubmed: 29457664
Structure. 2012 Dec 5;20(12):2138-50
pubmed: 23123110
Structure. 2018 Aug 7;26(8):1072-1079.e4
pubmed: 29937358
Chem Commun (Camb). 2013 Apr 7;49(27):2747-9
pubmed: 23338477
Nat Rev Mol Cell Biol. 2015 Jan;16(1):18-29
pubmed: 25531225
J Biomol NMR. 2009 Mar;43(3):145-50
pubmed: 19140010