Neutrophils do not impact viral load or the peak of disease severity during RSV infection.
Animals
Cells, Cultured
Chemokine CXCL1
Cytokines
/ metabolism
Disease Models, Animal
Humans
Inflammation Mediators
/ metabolism
Lung
/ immunology
Mice, Inbred C57BL
Negative Results
Neutrophils
/ immunology
Respiratory Syncytial Virus Infections
/ immunology
Respiratory Syncytial Virus, Human
Severity of Illness Index
Viral Load
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
24 01 2020
24 01 2020
Historique:
received:
24
05
2019
accepted:
07
01
2020
entrez:
26
1
2020
pubmed:
26
1
2020
medline:
15
12
2020
Statut:
epublish
Résumé
Lung and airway neutrophils are a hallmark of severe disease in infants with respiratory syncytial virus (RSV)-induced lower respiratory tract infections. Despite their abundance in the lungs during RSV infection of both mice and man, the role of neutrophils in viral control and in immune pathology is not clear. Here, antibody mediated neutrophil depletion was used to investigate the degree to which neutrophils impact the lung immune environment, the control of viral replication and the peak severity of disease after RSV infection of mice. Neutrophil depletion did not substantially affect the levels of inflammatory mediators such as type I interferons, IL-6, TNF-α or IL-1β in response to RSV. In addition, the lack of neutrophils did not change the viral load during RSV infection. Neither neutrophil depletion nor the enhancement of lung neutrophils by administration of the chemoattractant CXCL1 during RSV infection affected disease severity as measured by weight loss. Therefore, in this model of RSV infection, lung neutrophils do not offer obvious benefits to the host in terms of increasing anti-viral inflammatory responses or restricting viral replication and neutrophils do not contribute to disease severity.
Identifiants
pubmed: 31980667
doi: 10.1038/s41598-020-57969-w
pii: 10.1038/s41598-020-57969-w
pmc: PMC6981203
doi:
Substances chimiques
Chemokine CXCL1
0
Cytokines
0
Inflammation Mediators
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1110Subventions
Organisme : Wellcome Trust
Pays : United Kingdom
Organisme : Wellcome Trust (Wellcome)
ID : 109058/Z/15/Z
Pays : International
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