Plasma microRNA levels in male and female children with cystic fibrosis.

Child Child, Preschool Cystic Fibrosis / blood Female Gene Ontology Humans Infant Male MicroRNAs / blood Prognosis Reverse Transcriptase Polymerase Chain Reaction Sex Characteristics

Journal

Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288

Informations de publication

Date de publication:
24 01 2020
Historique:
received: 30 08 2019
accepted: 21 12 2019
entrez: 26 1 2020
pubmed: 26 1 2020
medline: 21 11 2020
Statut: epublish

Résumé

A gender gap exists in cystic fibrosis (CF). Here we investigate whether plasma microRNA expression profiles differ between the sexes in CF children. MicroRNA expression was quantified in paediatric CF plasma (n = 12; six females; Age range:1-6; Median Age: 3; 9 p.Phe508del homo- or heterozygotes) using TaqMan OpenArray Human miRNA Panels. Principal component analysis indicated differences in male versus female miRNA profiles. The miRNA array analysis revealed two miRNAs which were significantly increased in the female samples (miR-885-5p; fold change (FC):5.07, adjusted p value: 0.026 and miR-193a-5p; FC:2.6, adjusted p value: 0.031), although only miR-885-5p was validated as increased in females using specific qPCR assay (p < 0.0001). Gene ontology analysis of miR-885-5p validated targets identified cell migration, motility and fibrosis as processes potentially affected, with RAC1-mediated signalling featuring significantly. There is a significant increase in miR-885-5p in plasma of females versus males with CF under six years of age.

Identifiants

DOI: 10.1038/s41598-020-57964-1 PMID: 31980676 PMC: PMC6981182
pubmed: 31980676
doi: 10.1038/s41598-020-57964-1
pii: 10.1038/s41598-020-57964-1
pmc: PMC6981182
doi:

Substances chimiques

MIRN885 microRNA, human 0
MicroRNAs 0

Types de publication

Comparative Study Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

1141

Références

Lommatzsch, S. T. & Aris, R. Genetics of cystic fibrosis. Semin. Respir. Crit. Care Med. 30, 531–8 (2009).
pubmed: 19760540 doi: 10.1055/s-0029-1238911
Corey, M., Edwards, L., Levison, H. & Knowles, M. Longitudinal analysis of pulmonary function decline in patients with cystic fibrosis. J. Pediatr. 131, 809–14 (1997).
pubmed: 9427882 doi: 10.1016/S0022-3476(97)70025-8 pmcid: 9427882
Konstan, M. W. et al. Risk factors for rate of decline in forced expiratory volume in one second in children and adolescents with cystic fibrosis. J. Pediatr. 151, 134–9, 139.e1 (2007).
pubmed: 17643762 doi: 10.1016/j.jpeds.2007.03.006
Demko, C. A., Byard, P. J. & Davis, P. B. Gender differences in cystic fibrosis: Pseudomonas aeruginosa infection. J. Clin. Epidemiol. 48, 1041–9 (1995).
pubmed: 7775991 doi: 10.1016/0895-4356(94)00230-N
Levy, H. et al. Predictors of mucoid Pseudomonas colonization in cystic fibrosis patients. Pediatr. Pulmonol. 43, 463–71 (2008).
pubmed: 18361452 pmcid: 3693457 doi: 10.1002/ppul.20794
Sweezey, N. B. & Ratjen, F. The cystic fibrosis gender gap: potential roles of estrogen. Pediatr. Pulmonol. 49, 309–17 (2014).
pubmed: 24339235 doi: 10.1002/ppul.22967
Harness-Brumley, C. L., Elliott, A. C., Rosenbluth, D. B., Raghavan, D. & Jain, R. Gender Differences in Outcomes of Patients with Cystic Fibrosis. J. Women’s Heal. 23, 1012–1020 (2014).
doi: 10.1089/jwh.2014.4985
Olesen, H. V. et al. Gender differences in the Scandinavian cystic fibrosis population. Pediatr. Pulmonol. 45, 959–965 (2010).
pubmed: 20672360 doi: 10.1002/ppul.21265
McIntyre, K. Gender and survival in cystic fibrosis. Curr. Opin. Pulm. Med. 19, 692–697 (2013).
pubmed: 24048080 doi: 10.1097/MCP.0b013e328365af49
Sohel, M. H. Extracellular/Circulating MicroRNAs: Release Mechanisms, Functions and Challenges. Achiev. Life Sci. 10, 175–186 (2016).
Chem, J. B. et al. A Transfer of miRNA Power: Secretory Mechanisms and Intercellular Transfer of MicroRNAs in Living Cells. J. Biol. Chem. 285, e99946–e99946 (2010).
doi: 10.1074/jbc.P110.107821
Vickers, K. C., Palmisano, B. T., Shoucri, B. M., Shamburek, R. D. & Remaley, A. T. MicroRNAs are transported in plasma and delivered to recipient cells by high-density lipoproteins. Nat. Cell Biol. 13, 423–435 (2011).
pubmed: 21423178 pmcid: 3074610 doi: 10.1038/ncb2210
Cui, M. et al. Circulating MicroRNAs in Cancer: Potential and Challenge. Front. Genet. 10 (2019).
Glasgow, A. M. A., Santi, C. D & Greene, C. M. Non-coding RNA in cystic fibrosis. Biochem. Soc. Trans. BST20170469, https://doi.org/10.1042/BST20170469 (2018).
pubmed: 29743276 doi: 10.1042/BST20170469 pmcid: 29743276
Mooney, C. et al. High Throughput qPCR Expression Profiling of Circulating MicroRNAs Reveals Minimal Sex- and Sample Timing-Related Variation in Plasma of Healthy Volunteers. PLoS One 10, e0145316 (2015).
pubmed: 26699132 pmcid: 4689368 doi: 10.1371/journal.pone.0145316
Wang, K. et al. Comparing the MicroRNA spectrum between serum and plasma. PLoS One 7, e41561 (2012).
pubmed: 22859996 pmcid: 22859996 doi: 10.1371/journal.pone.0041561
Blondal, T. et al. Assessing sample and miRNA profile quality in serum and plasma or other biofluids. Methods 59, S1–S6 (2013).
pubmed: 23036329 doi: 10.1016/j.ymeth.2012.09.015
Weber, J. A. et al. The microRNA spectrum in 12 body fluids. Clin. Chem. 56, 1733–41 (2010).
pubmed: 20847327 pmcid: 4846276 doi: 10.1373/clinchem.2010.147405
Chen, X. et al. Characterization of microRNAs in serum: a novel class of biomarkers for diagnosis of cancer and other diseases. Cell Res. 18, 997–1006 (2008).
pubmed: 18766170 doi: 10.1038/cr.2008.282
Mitchell, P. S. et al. Circulating microRNAs as stable blood-based markers for cancer detection. Proc. Natl. Acad. Sci. USA 105, 10513–8 (2008).
pubmed: 18663219 doi: 10.1073/pnas.0804549105
Haider, B. A. et al. A critical evaluation of microRNA biomarkers in non-neoplastic disease. PLoS One 9, e89565 (2014).
pubmed: 24586876 pmcid: 3935874 doi: 10.1371/journal.pone.0089565
Oglesby, I. K. et al. miR-126 Is Downregulated in Cystic Fibrosis Airway Epithelial Cells and Regulates TOM1 Expression. J. Immunol. 184, 1702–1709 (2010).
pubmed: 20083669 doi: 10.4049/jimmunol.0902669
Bhattacharyya, S. et al. Elevated miR-155 promotes inflammation in cystic fibrosis by driving hyperexpression of interleukin-8. J. Biol. Chem. 286, 11604–15 (2011).
pubmed: 21282106 pmcid: 3064214 doi: 10.1074/jbc.M110.198390
Gui, J. et al. Serum microRNA characterization identifies miR - 885 - 5p as a potential marker for detecting liver pathologies. Clin. Sci. 120, 183–193 (2011).
pubmed: 20815808 doi: 10.1042/CS20100297
Vencken, S. F., Greene, C. M. & McKiernan, P. J. Non-coding RNA as lung disease biomarkers. Thorax 70, 501–3 (2015).
pubmed: 25550385 doi: 10.1136/thoraxjnl-2014-206193
Krause, K. et al. The expression of Mirc1/Mir17–92 cluster in sputum samples correlates with pulmonary exacerbations in cystic fibrosis patients. J. Cyst. Fibros. https://doi.org/10.1016/j.jcf.2017.11.005 (2017).
doi: 10.1016/j.jcf.2017.11.005 pubmed: 29241629 pmcid: 5995663
Cook, N. L., Pereira, T. N., Lewindon, P. J., Shepherd, R. W. & Ramm, G. A. Circulating microRNAs as noninvasive diagnostic biomarkers of liver disease in children with cystic fibrosis. J. Pediatr. Gastroenterol. Nutr. 60, 247–54 (2015).
pubmed: 25625579 doi: 10.1097/MPG.0000000000000600
Ideozu, J. E., Zhang, X., Rangaraj, V., McColley, S. & Levy, H. Microarray profiling identifies extracellular circulating miRNAs dysregulated in cystic fibrosis. Sci. Rep. 9 (2019).
Cheng, J., Wang, H. & Guggino, W. B. Regulation of cystic fibrosis transmembrane regulator trafficking and protein expression by a Rho family small GTPase TC10. J. Biol. Chem. 280, 3731–9 (2005).
pubmed: 15546864 doi: 10.1074/jbc.M410026200
Panganiban, R. A. M. & Day, R. M. Hepatocyte growth factor in lung repair and pulmonary fibrosis. Acta Pharmacol. Sin. 32, 12–20 (2011).
pubmed: 21131996 doi: 10.1038/aps.2010.90 pmcid: 21131996
Moniz, S. et al. HGF Stimulation of Rac1 Signaling Enhances Pharmacological Correction of the Most Prevalent Cystic Fibrosis Mutant F508del-CFTR. ACS Chem. Biol. 8, 432–442 (2013).
pubmed: 23148778 doi: 10.1021/cb300484r pmcid: 23148778
Loureiro, C. A. et al. A molecular switch in the scaffold NHERF1 enables misfolded CFTR to evade the peripheral quality control checkpoint. Sci. Signal. 8, ra48 (2015).
pubmed: 25990958 doi: 10.1126/scisignal.aaa1580 pmcid: 25990958
Matos, A. M. et al. Prolonged co-treatment with HGF sustains epithelial integrity and improves pharmacological rescue of Phe508del-CFTR. Sci. Rep. 8, 13026 (2018).
pubmed: 30158635 pmcid: 6115363 doi: 10.1038/s41598-018-31514-2
Oglesby, I. K. et al. miR-17 overexpression in cystic fibrosis airway epithelial cells decreases interleukin-8 production. Eur. Respir. J. 46, 1350–1360 (2015).
pubmed: 26160865 doi: 10.1183/09031936.00163414 pmcid: 26160865
Vencken, S. et al. Nebulised lipid–polymer hybrid nanoparticles for the delivery of a therapeutic anti-inflammatory microRNA to bronchial epithelial cells. ERJ Open Res. 5, 00161–02018 (2019).
pubmed: 30972350 pmcid: 6452044 doi: 10.1183/23120541.00161-2018
Bardin, P. et al. Small RNA and transcriptome sequencing reveal the role of miR-199a-3p in inflammatory processes in cystic fibrosis airways. J. Pathol. 245, 410–420 (2018).
pubmed: 29732561 doi: 10.1002/path.5095 pmcid: 29732561
Liu, X. et al. MicroRNA in vivo precipitation identifies miR-151-3p as a computational unpredictable miRNA to target Stat3 and inhibits innate IL-6 production. Cell. Mol. Immunol. 15, 99–110 (2018).
pubmed: 28890541 doi: 10.1038/cmi.2017.82 pmcid: 28890541
Xie, T. et al. MicroRNA-29c Prevents Pulmonary Fibrosis by Regulating Epithelial Cell Renewal and Apoptosis. Am. J. Respir. Cell Mol. Biol. 57, 721–732 (2017).
pubmed: 28799781 pmcid: 5765420 doi: 10.1165/rcmb.2017-0133OC
Wang, L. et al. The miR-29c-KIAA1199 axis regulates gastric cancer migration by binding with WBP11 and PTP4A3. Oncogene, https://doi.org/10.1038/s41388-018-0642-0 (2019).
pubmed: 30626935 doi: 10.1038/s41388-018-0642-0 pmcid: 30626935
Renwick, J. et al. The microbial community of the cystic fibrosis airway is disrupted in early life. PLoS One 9, e109798 (2014).
pubmed: 25526264 pmcid: 4272276 doi: 10.1371/journal.pone.0109798
Huber, W. et al. Orchestrating high-throughput genomic analysis with Bioconductor. Nat. Methods 12, 115–21 (2015).
pubmed: 25633503 pmcid: 4509590 doi: 10.1038/nmeth.3252
McCall, M. N., McMurray, H. R., Land, H. & Almudevar, A. On non-detects in qPCR data. Bioinformatics 30, 2310–2316 (2014).
pubmed: 24764462 pmcid: 4133581 doi: 10.1093/bioinformatics/btu239
Dvinge, H. & Bertone, P. HTqPCR: high-throughput analysis and visualization of quantitative real-time PCR data in R. Bioinformatics 25, 3325–3326 (2009).
pubmed: 19808880 pmcid: 2788924 doi: 10.1093/bioinformatics/btp578
Benjamini, Y. & Hochberg, Y. Controlling the False Discovery Rate: A Practical and Powerful Approach to Multiple Testing. Journal of the Royal Statistical Society. Series B (Methodological) 57, 289–300 (1995).
doi: 10.1111/j.2517-6161.1995.tb02031.x
Haunsberger, S. J., Connolly, N. M. C. & Prehn, J. H. M. miRNAmeConverter: an R/bioconductor package for translating mature miRNA names to different miRBase versions. Bioinformatics 33, btw660 (2016).
doi: 10.1093/bioinformatics/btw660
Chou, C.-H. et al. miRTarBase 2016: updates to the experimentally validated miRNA-target interactions database. Nucleic Acids Res. 44, D239–47 (2016).
pubmed: 26590260 doi: 10.1093/nar/gkv1258
Kuleshov, M. V. et al. Enrichr: a comprehensive gene set enrichment analysis web server 2016 update. Nucleic Acids Res. 44, W90–7 (2016).
pubmed: 27141961 pmcid: 4987924 doi: 10.1093/nar/gkw377
Supek, F., Bošnjak, M., Škunca, N. & Šmuc, T. REVIGO summarizes and visualizes long lists of gene ontology terms. PLoS One 6, e21800 (2011).
pubmed: 21789182 pmcid: 3138752 doi: 10.1371/journal.pone.0021800

Auteurs

C Mooney (C)

Department of Physiology and Medical Physics, Royal College of Surgeons in Ireland, Dublin, Ireland.
School of Computer Science, University College Dublin, Dublin, Ireland.

P J McKiernan (PJ)

Lung Biology Group, Department of Clinical Microbiology, Royal College of Surgeons in Ireland, Dublin, Ireland.
ORCID: 0000-0001-6756-0799

R Raoof (R)

Department of Physiology and Medical Physics, Royal College of Surgeons in Ireland, Dublin, Ireland.
Department of Anatomy, College of Medicine, University of Mosul, Mosul, Iraq.

D C Henshall (DC)

Department of Physiology and Medical Physics, Royal College of Surgeons in Ireland, Dublin, Ireland.
FutureNeuro Research Centre, RCSI, Dublin, Ireland.

B Linnane (B)

Study for Host Infection in Early Lung Disease in CF (SHIELD CF), National Children's Research Centre, Children's Health Ireland at Crumlin, Dublin, Ireland.
Graduate Entry Medical School and Centre for Interventions in Infection, Inflammation & Immunity (4i), University of Limerick, Limerick, Ireland.

P McNally (P)

Study for Host Infection in Early Lung Disease in CF (SHIELD CF), National Children's Research Centre, Children's Health Ireland at Crumlin, Dublin, Ireland.
Department of Paediatrics, Royal College of Surgeons in Ireland, Dublin, Ireland.

A M A Glasgow (AMA)

Lung Biology Group, Department of Clinical Microbiology, Royal College of Surgeons in Ireland, Dublin, Ireland. arleneglasgow@rcsi.ie.

C M Greene (CM)

Lung Biology Group, Department of Clinical Microbiology, Royal College of Surgeons in Ireland, Dublin, Ireland.

Articles similaires

[Redispensing of expensive oral anticancer medicines: a practical application].

Lisanne N van Merendonk, Kübra Akgöl, Bastiaan Nuijen
1.00
Humans Antineoplastic Agents Administration, Oral Drug Costs Counterfeit Drugs

Smoking Cessation and Incident Cardiovascular Disease.

Jun Hwan Cho, Seung Yong Shin, Hoseob Kim et al.
1.00
Humans Male Smoking Cessation Cardiovascular Diseases Female

Evaluation of Low-Value Services Across Major Medicare Advantage Insurers and Traditional Medicare.

Ciara Duggan, Adam L Beckman, Ishani Ganguli et al.
1.00
Humans United States Aged Cross-Sectional Studies Medicare Part C

Effectiveness of Virtual Yoga for Chronic Low Back Pain: A Randomized Clinical Trial.

Hallie Tankha, Devyn Gaskins, Amanda Shallcross et al.
1.00
Humans Yoga Low Back Pain Female Male

Classifications MeSH

questionsmedicales.fr › Personnes › Tranches d'âge › Enfant › Plasma microRNA levels in male and female...
questionsmedicales.fr › Personnes › Tranches d'âge › Enfant › Enfant d'âge préscolaire › Plasma microRNA levels in male and female...
questionsmedicales.fr › Malformations et maladies congénitales, héréditaires et néonatales › Maladies néonatales › Mucoviscidose › Plasma microRNA levels in male and female...
questionsmedicales.fr › Sciences de l'information › Sources d'information › Services d'information › Documentation › Vocabulaire contrôlé › Ontologies biologiques › Gene Ontology › Plasma microRNA levels in male and female...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Plasma microRNA levels in male and female...
questionsmedicales.fr › Personnes › Tranches d'âge › Nourrisson › Plasma microRNA levels in male and female...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ARN › ARN non traduit › Petit ARN non traduit › microARN › Plasma microRNA levels in male and female...
questionsmedicales.fr › Diagnostic › Pronostic › Plasma microRNA levels in male and female...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Techniques d'amplification d'acides nucléiques › Réaction de polymérisation en chaîne › RT-PCR › Plasma microRNA levels in male and female...
questionsmedicales.fr › Phénomènes physiologiques des appareils urinaire et reproducteur › Phénomènes physiologiques de la reproduction › Caractères sexuels › Plasma microRNA levels in male and female...