Antibacterial and immunoregulatory activity of a novel hepcidin homologue in diploid hybrid fish (Carassius auratus cuvieri ♀ × Carassius auratus red var ♂).
Aeromonas hydrophila
Diploid hybrid fish
Gene expression
Hepcidin
Journal
Fish & shellfish immunology
ISSN: 1095-9947
Titre abrégé: Fish Shellfish Immunol
Pays: England
ID NLM: 9505220
Informations de publication
Date de publication:
Mar 2020
Mar 2020
Historique:
received:
05
10
2019
revised:
16
01
2020
accepted:
19
01
2020
pubmed:
26
1
2020
medline:
4
11
2020
entrez:
26
1
2020
Statut:
ppublish
Résumé
Hepcidin, a multifunctional hormone oligopeptide, not only exhibits a regulatory role in iron metabolism, but also participates in the regulation of teleostean immunity. In this study, ORF sequence of WR-hepcidin was 258 bp and encoded 85 amino acid residues. Tissue-specific analysis revealed that the highest expression of WR-hepcidin was observed in liver. Aeromonas hydrophila challenge can sharply increased WR-hepcidin mRNA expression in liver, trunk kidney and spleen. The purified WR-hepcidin fusion peptide can directly bind to A. hydrophila and Streptococcus agalactiae, reduce the relative bacterial activity, limit bacterial growth and attenuate their dissemination to tissues in vivo. In addition, the treatment of WR-hepcidin fusion protein can diminish the production of pro-inflammatory cytokines. These results indicated that WR-hepcidin can play a negative regulatory role in bacteria-stimulated pro-inflammatory cytokines production and MyD88-IRAK4 activation.
Identifiants
pubmed: 31981776
pii: S1050-4648(20)30038-3
doi: 10.1016/j.fsi.2020.01.038
pii:
doi:
Substances chimiques
Fish Proteins
0
Hepcidins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
551-563Commentaires et corrections
Type : ErratumIn
Informations de copyright
Copyright © 2020 Elsevier Ltd. All rights reserved.