Enzyme-responsive Fluorescent Ionic Liquid.
Alkaline phosphatase
enzyme activity assay
ionic liquid
optical sensor
plasticized PVC membrane
Journal
Analytical sciences : the international journal of the Japan Society for Analytical Chemistry
ISSN: 1348-2246
Titre abrégé: Anal Sci
Pays: Switzerland
ID NLM: 8511078
Informations de publication
Date de publication:
10 Feb 2020
10 Feb 2020
Historique:
pubmed:
28
1
2020
medline:
2
10
2020
entrez:
28
1
2020
Statut:
ppublish
Résumé
Herein, we describe the development of a novel material, "enzyme-responsive fluorescent ionic liquid", which enabled a highly sensitive detection of alkaline phosphatase (ALP). We prepared a plasticized poly(vinyl chloride) (PVC) membrane using this new material as a plasticizer and quantified ALP in aqueous solutions. Preliminary results suggested that the PVC membrane responded to ALP at an interface between the membrane and the sample solution with anion extraction to maintain electroneutrality in the membrane phase. The developed PVC membrane showed an approximately six-times higher sensitivity than the conventional membrane, thereby demonstrating highly sensitive ALP detection. These results suggested the potential applicability of the proposed membrane for highly sensitive protein detection by using ALP-labeled antibodies.
Identifiants
pubmed: 31983712
doi: 10.2116/analsci.19C013
pii: 10.2116/analsci.19C013
doi:
Substances chimiques
Ionic Liquids
0
Membranes, Artificial
0
Water
059QF0KO0R
Polyvinyl Chloride
9002-86-2
Alkaline Phosphatase
EC 3.1.3.1
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
143-145Références
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