Immunological and oxidative stress biomarkers in Ankylosing Spondylitis patients with or without metabolic syndrome.
Adiponectin
/ immunology
Adult
Biomarkers
/ metabolism
Cytokines
/ immunology
Female
Humans
Inflammation
/ immunology
Interleukin-1beta
/ immunology
Male
Metabolic Syndrome
/ immunology
MicroRNAs
/ immunology
NF-kappa B
/ immunology
Oxidative Stress
/ immunology
Spondylitis, Ankylosing
/ immunology
Th17 Cells
/ immunology
Transcription Factors
/ immunology
Tumor Necrosis Factor-alpha
/ immunology
Ankylosing Spondylitis
Immunological factors
Metabolic syndrome
Oxidative stress
Journal
Cytokine
ISSN: 1096-0023
Titre abrégé: Cytokine
Pays: England
ID NLM: 9005353
Informations de publication
Date de publication:
04 2020
04 2020
Historique:
received:
20
07
2019
revised:
15
01
2020
accepted:
17
01
2020
pubmed:
28
1
2020
medline:
26
8
2021
entrez:
28
1
2020
Statut:
ppublish
Résumé
Ankylosing Spondylitis (AS) is a chronic inflammatory disorder of the spine and sacroiliac joints with unidentified etiology closely associated with metabolic syndrome (MetS). Recent studies have reported that immunological and oxidative stress factors are implicated in AS pathogenesis. The aim of this study was to investigate the oxidative and immunological factors in AS patients with or without MetS compare to control group. Real-Time PCR measured expression level of cytokines, transcription factors and related miRNAs. In addition, Th17 and Treg frequencies and cytokines secretion were evaluated by flowcytometry and ELISA methods, respectively. The oxidative stress biomarkers were also assessed with biochemical methods. In AS patients with MetS, higher Th17 and lower Treg frequency were observed. Increased levels of NF-kB and AP-1 mRNA expression were seen in AS patients with MetS (p = 0.0263 and p = 0.0104, respectively). MiR-146a and miR-223 were significantly decreased (p = 0.0005, p = 0.0161, respectively) and increase in miR-21 (p = 0.0002) was observed in AS patients with MetS compared to AS patients without MetS. Additionally, the secretion of TNF-α (p = 0.0167), IL-1β (p = 0.303), CCL2 (p = 0.0254), CCL3 (p = 0.0119), CXCL8 (p = 0.0364), adiponectin (p = 0.0183) and the levels of SOD (p = 0.0421), NO (p = 0.0451) and CAT (p = 0.0128) were increased in AS patients with MetS. We were not observed significant differences in TOS and GPX levels between studied groups. The higher levels of oxidative stress and immunological inflammatory markers in AS patients with MetS provide further evidences on the oxidative stress and immunological relationship in these patients.
Identifiants
pubmed: 31986444
pii: S1043-4666(20)30018-1
doi: 10.1016/j.cyto.2020.155002
pii:
doi:
Substances chimiques
Adiponectin
0
Biomarkers
0
Cytokines
0
Interleukin-1beta
0
MicroRNAs
0
NF-kappa B
0
Transcription Factors
0
Tumor Necrosis Factor-alpha
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
155002Informations de copyright
Copyright © 2020 Elsevier Ltd. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.