Whole-genome fingerprint of the DNA methylome during chemically induced differentiation of the human AML cell line HL-60/S4.
Cell Differentiation
/ drug effects
Computational Biology
/ methods
CpG Islands
DNA Methylation
Enhancer Elements, Genetic
Epigenome
Epigenomics
/ methods
Gene Expression Profiling
Gene Expression Regulation, Leukemic
HL-60 Cells
Humans
Leukemia, Myeloid, Acute
/ drug therapy
Molecular Sequence Annotation
Promoter Regions, Genetic
DNA methylation
Differentiation
Epigenomic regulation
HL60
Long range interactions
Promyelocyte
Journal
Biology open
ISSN: 2046-6390
Titre abrégé: Biol Open
Pays: England
ID NLM: 101578018
Informations de publication
Date de publication:
17 02 2020
17 02 2020
Historique:
pubmed:
29
1
2020
medline:
29
1
2020
entrez:
29
1
2020
Statut:
epublish
Résumé
Epigenomic regulation plays a vital role in cell differentiation. The leukemic HL-60/S4 [human myeloid leukemic cell line HL-60/S4 (ATCC CRL-3306)] promyelocytic cell can be easily differentiated from its undifferentiated promyelocyte state into neutrophil- and macrophage-like cell states. In this study, we present the underlying genome and epigenome architecture of HL-60/S4 through its differentiation. We performed whole-genome bisulphite sequencing of HL-60/S4 cells and their differentiated counterparts. With the support of karyotyping, we show that HL-60/S4 maintains a stable genome throughout differentiation. Analysis of differential Cytosine-phosphate-Guanine dinucleotide methylation reveals that most methylation changes occur in the macrophage-like state. Differential methylation of promoters was associated with immune-related terms. Key immune genes,
Identifiants
pubmed: 31988093
pii: bio.044222
doi: 10.1242/bio.044222
pmc: PMC7044446
pii:
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Informations de copyright
© 2020. Published by The Company of Biologists Ltd.
Déclaration de conflit d'intérêts
Competing interestsThe authors declare no competing or financial interests.
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