Sustained-immunostimulatory nanocellulose scaffold to enhance vaccine efficacy.
Adjuvants, Immunologic
/ chemistry
Animals
Antigens
/ administration & dosage
Cellulose
/ chemistry
Delayed-Action Preparations
/ chemistry
Female
Immunity, Cellular
/ drug effects
Mice
Mice, Inbred C57BL
RAW 264.7 Cells
Tissue Scaffolds
/ chemistry
Vaccination
/ methods
Vaccines
/ administration & dosage
drug delivery
hydrogel
immune scaffold
nanocellulose
vaccine
Journal
Journal of biomedical materials research. Part A
ISSN: 1552-4965
Titre abrégé: J Biomed Mater Res A
Pays: United States
ID NLM: 101234237
Informations de publication
Date de publication:
05 2020
05 2020
Historique:
received:
20
08
2019
revised:
06
01
2020
accepted:
10
01
2020
pubmed:
29
1
2020
medline:
13
10
2021
entrez:
29
1
2020
Statut:
ppublish
Résumé
An implantable scaffold-based vaccination system is a promising platform to generate robust immune responses by modulating the immune system. However, establishment of an effective vaccine using a biodegradable, cell-infiltrative scaffold remain challenging. Here we demonstrate a biodegradable, nanocellulose-based immune scaffold capable of sustainably activating immune cells to elicit cellular immunity. Cell-infiltrative nanocellulose hydrogels were used as a delivery carrier and cellular scaffold microenvironment. Nanofibrous hydrogels allowed for high cell infiltration and delivery of antigen-loaded nanocellulose while cells degraded the hydrogel matrix. Importantly, antigen-loaded nanocellulose hydrogels exhibited sustained activation of macrophages in vitro compared to free antigen and collagen scaffold. Histological observation revealed infiltration of macrophages and dendritic cells into the nanocellulose scaffold subcutaneously implanted in mice. In vivo fluorescence imaging indicated that the implanted scaffold released antigens at a zero-order release profile without burst diffusion. Antigen-loaded nanocellulose hydrogels increased interferon-γ-producing cells compared to free antigen injection, suggesting the enhancement of cellular immunity. Thus, nanocellulose immune scaffold may serve as a sustained-immunostimulatory vaccine platform by providing favorable microenvironments for immune cells thus enhancing vaccine efficacy.
Substances chimiques
Adjuvants, Immunologic
0
Antigens
0
Delayed-Action Preparations
0
Vaccines
0
Cellulose
9004-34-6
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1159-1170Informations de copyright
© 2020 Wiley Periodicals, Inc.
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