Cell cycle oscillators underlying orderly proteolysis of E2F8.
Amino Acid Motifs
Apc1 Subunit, Anaphase-Promoting Complex-Cyclosome
/ metabolism
Cell Cycle
/ physiology
Cell-Free System
Cyclins
/ metabolism
E2F1 Transcription Factor
/ metabolism
G1 Phase
/ physiology
G2 Phase
/ physiology
HeLa Cells
Humans
Mitosis
/ physiology
Phosphorylation
Protein Processing, Post-Translational
Proteolysis
Recombinant Proteins
/ metabolism
Repressor Proteins
/ metabolism
Ubiquitination
Journal
Molecular biology of the cell
ISSN: 1939-4586
Titre abrégé: Mol Biol Cell
Pays: United States
ID NLM: 9201390
Informations de publication
Date de publication:
01 04 2020
01 04 2020
Historique:
pubmed:
30
1
2020
medline:
3
6
2021
entrez:
30
1
2020
Statut:
ppublish
Résumé
E2F8 is a transcriptional repressor that antagonizes E2F1 at the crossroads of the cell cycle, apoptosis, and cancer. Previously, we discovered that E2F8 is a direct target of the APC/C ubiquitin ligase. Nevertheless, it remains unknown how E2F8 is dynamically controlled throughout the entirety of the cell cycle. Here, using newly developed human cell-free systems that recapitulate distinct inter-mitotic and G1 phases and a continuous transition from prometaphase to G1, we reveal an interlocking dephosphorylation switch coordinating E2F8 degradation with mitotic exit and the activation of APC/C
Identifiants
pubmed: 31995441
doi: 10.1091/mbc.E19-12-0725
pmc: PMC7185961
doi:
Substances chimiques
ANAPC1 protein, human
0
Apc1 Subunit, Anaphase-Promoting Complex-Cyclosome
0
CCNF protein, human
0
Cyclins
0
E2F1 Transcription Factor
0
E2F1 protein, human
0
E2F8 protein, human
0
Recombinant Proteins
0
Repressor Proteins
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
725-740Subventions
Organisme : NCI NIH HHS
ID : P30 CA016086
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM120309
Pays : United States
Organisme : NIGMS NIH HHS
ID : T32 GM007040
Pays : United States
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