LiCoO
Alveolar Epithelial Cells
/ drug effects
Animals
Bronchoalveolar Lavage Fluid
/ chemistry
Cell Survival
/ drug effects
Cells, Cultured
Cobalt
/ chemistry
DNA Damage
Electric Power Supplies
Female
Hydroxyl Radical
/ metabolism
Lung
/ drug effects
Micronuclei, Chromosome-Defective
/ chemically induced
Oxides
/ chemistry
Particle Size
Particulate Matter
/ chemistry
Rats
Rats, Wistar
Comet assay
Genotoxicity
Micronucleus assay
Journal
Particle and fibre toxicology
ISSN: 1743-8977
Titre abrégé: Part Fibre Toxicol
Pays: England
ID NLM: 101236354
Informations de publication
Date de publication:
29 01 2020
29 01 2020
Historique:
received:
06
08
2019
accepted:
15
01
2020
entrez:
31
1
2020
pubmed:
31
1
2020
medline:
15
12
2020
Statut:
epublish
Résumé
Li-ion batteries (LIB) are used in most portable electronics. Among a wide variety of materials, LiCoO We assessed the mutagenic potential of LCO and LTO particles in vitro by performing a cytokinesis-block micronucleus (MN) assay with rat lung epithelial cells (RLE), as well as in vivo in alveolar type II epithelial (AT-II) cells. LCO particles induced MN in vitro at non-cytotoxic concentrations and in vivo at non-inflammatory doses, indicating a primary genotoxic mechanism. LTO particles did not induce MN. Electron paramagnetic resonance and terephthalate assays showed that LCO particles produce hydroxyl radicals (•OH). Catalase inhibits this •OH production. In an alkaline comet assay with the oxidative DNA damage repair enzyme human 8-oxoguanine DNA glycosylase 1, LCO particles induced DNA strand breaks and oxidative lesions. The addition of catalase reduced the frequency of MN induced by LCO particles in vitro. We report the mutagenic activity of LCO particles used in LIB in vitro and in vivo. Our data support the role of Co(II) ions released from these particles in their primary genotoxic activity which includes the formation of •OH by a Fenton-like reaction, oxidative DNA lesions and strand breaks, thus leading to chromosomal breaks and the formation of MN. Documenting the genotoxic potential of the other LIB particles, especially those containing Co and/or Ni, is therefore needed to guarantee a safe and sustainable development of LIB.
Sections du résumé
BACKGROUND
Li-ion batteries (LIB) are used in most portable electronics. Among a wide variety of materials, LiCoO
RESULTS
We assessed the mutagenic potential of LCO and LTO particles in vitro by performing a cytokinesis-block micronucleus (MN) assay with rat lung epithelial cells (RLE), as well as in vivo in alveolar type II epithelial (AT-II) cells. LCO particles induced MN in vitro at non-cytotoxic concentrations and in vivo at non-inflammatory doses, indicating a primary genotoxic mechanism. LTO particles did not induce MN. Electron paramagnetic resonance and terephthalate assays showed that LCO particles produce hydroxyl radicals (•OH). Catalase inhibits this •OH production. In an alkaline comet assay with the oxidative DNA damage repair enzyme human 8-oxoguanine DNA glycosylase 1, LCO particles induced DNA strand breaks and oxidative lesions. The addition of catalase reduced the frequency of MN induced by LCO particles in vitro.
CONCLUSIONS
We report the mutagenic activity of LCO particles used in LIB in vitro and in vivo. Our data support the role of Co(II) ions released from these particles in their primary genotoxic activity which includes the formation of •OH by a Fenton-like reaction, oxidative DNA lesions and strand breaks, thus leading to chromosomal breaks and the formation of MN. Documenting the genotoxic potential of the other LIB particles, especially those containing Co and/or Ni, is therefore needed to guarantee a safe and sustainable development of LIB.
Identifiants
pubmed: 31996255
doi: 10.1186/s12989-020-0338-9
pii: 10.1186/s12989-020-0338-9
pmc: PMC6990559
doi:
Substances chimiques
Oxides
0
Particulate Matter
0
lithium cobalt oxide
0
Hydroxyl Radical
3352-57-6
Cobalt
3G0H8C9362
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
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