Complexes of glucose oxidase with chitosan and dextran possessing enhanced stability.
Aldehydes
/ chemistry
Aspergillus niger
/ enzymology
Calcium Chloride
/ pharmacology
Chitosan
/ chemistry
Dextran Sulfate
/ chemistry
Drug Storage
Dynamic Light Scattering
Enzyme Activation
/ drug effects
Enzyme Stability
Ethanol
/ pharmacology
Glucose Oxidase
/ chemistry
Particle Size
Sodium Chloride
/ pharmacology
Spectrophotometry, Ultraviolet
Temperature
Chitosan
dextran
enzyme modification
enzyme polymer complexes
glucose oxidase
Journal
Preparative biochemistry & biotechnology
ISSN: 1532-2297
Titre abrégé: Prep Biochem Biotechnol
Pays: England
ID NLM: 9607037
Informations de publication
Date de publication:
2020
2020
Historique:
pubmed:
1
2
2020
medline:
9
2
2021
entrez:
1
2
2020
Statut:
ppublish
Résumé
In this study, the different mole ratios of glucose oxidase/chitosan/dextran-aldehyde and glucose oxidase/chitosan/dextran-sulfate complexes were synthesized. The modification of glucose oxidase by non-covalent complexation with dextran and chitosan in different molar ratios was studied in order to increase the enzyme activity. The enzyme/polymer complexes obtained were investigated by UV spectrophotometer and dynamic light scattering. Activity determination of synthesized complexes and free enzyme were performed at a temperature range. The best results were obtained by
Identifiants
pubmed: 32003292
doi: 10.1080/10826068.2020.1719515
doi:
Substances chimiques
Aldehydes
0
Ethanol
3K9958V90M
Sodium Chloride
451W47IQ8X
Chitosan
9012-76-4
Dextran Sulfate
9042-14-2
Glucose Oxidase
EC 1.1.3.4
Calcium Chloride
M4I0D6VV5M
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM