Diverse aminoglycoside phosphotransferase types conferring aminoglycoside resistance in
Aminoglycoside resistance
Escherichia coli
aminoglycoside phosphotransferase
antibiotic resistance
Journal
Indian journal of medical microbiology
ISSN: 1998-3646
Titre abrégé: Indian J Med Microbiol
Pays: United States
ID NLM: 8700903
Informations de publication
Date de publication:
Historique:
entrez:
1
2
2020
pubmed:
1
2
2020
medline:
2
6
2020
Statut:
ppublish
Résumé
The present study investigates the molecular basis of aph-mediated aminoglycoside resistance and their transmission dynamics in a tertiary care hospital of Northeast India. Two hundred forty one isolates (230 Escherichia coli and 11 Klebsiella pneumoniae) were collected and screened for aminoglycoside resistance genes. Various aph types were amplified using polymerase chain reaction (PCR) assay. Plasmid incompatibilty, horizontal transferability and ERIC-PCR based typing were carried out for all the positive isolates. Among them, 67 isolates showed the presence of aph gene. Aph (3")-IIIa and aph (3')-Via were predominant and horizontally transferable. All the plasmids were of incompatibility I1 group. Twenty-eight different haplotypes of E. coli were found harbouring aph gene types. This study was able to identify diverse aph types in a single centre and their corresponding phenotypic trait.
Identifiants
pubmed: 32003343
pii: IndianJMedMicrobiol_2019_37_3_418_274088
doi: 10.4103/ijmm.IJMM_19_262
doi:
Substances chimiques
Aminoglycosides
0
Anti-Bacterial Agents
0
Kanamycin Kinase
EC 2.7.1.95
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
418-422Déclaration de conflit d'intérêts
None