New insights on evolutionary aspects of Pythium insidiosum and other peronosporaleans.
Peronosporaleans
Pythium insidiosum
evolution story
mitochondrial gene
molecular clock
multi-locus phylogenetic approach
nuclear genes
oomycetes
Journal
Mycoses
ISSN: 1439-0507
Titre abrégé: Mycoses
Pays: Germany
ID NLM: 8805008
Informations de publication
Date de publication:
Apr 2020
Apr 2020
Historique:
received:
29
11
2019
revised:
27
01
2020
accepted:
28
01
2020
pubmed:
6
2
2020
medline:
17
9
2020
entrez:
4
2
2020
Statut:
ppublish
Résumé
The evolution of pathogenic mechanisms is a major challenge, which requires a thorough comprehension of the phylogenetic relationships of pathogens. Peronosporaleans encompasses a heterogeneous group of oomycetes that includes some animal/human pathogens, like Pythium insidiosum. We analysed here the phylogenetic positioning and other evolutionary aspects related to this species and other peronosporaleans, using a multi-locus approach with one mitochondrial and three nuclear genes. Phylogenetic patterns of 55 oomycetes were inferred by maximum likelihood and Bayesian analysis, and a relaxed molecular clock method was applied to infer the divergence time of some peronosporaleans branches. Pythium insidiosum was monophyletic with a major and polytomous clade of American isolates; however, Pythium spp. was found to be paraphyletic with Phytopythium sp. and Phytophthora spp. In general, peronosporaleans subdivided into four lineages, one of which evidenced a close relationship of P insidiosum, P aphanidermatum and P arrhenomanes. This lineage diverged about 63 million years ago (Mya), whereas P insidiosum diversified at approximately 24 Mya. The divergence of American and Thai isolates seems to have occurred at approximately 17 Mya, with further American diversification at 2.4 Mya. Overall, this study clarifies the phylogenetic relationships of P insidiosum regarding other peronosporaleans in a multi-locus perspective, despite previous claims that phylogenomic analyses are needed to accurately infer the patterns and processes related to the evolution of different lineages in this group. Additionally, this is the first time that a molecular clock was applied to study the evolution of P insidiosum.
Sections du résumé
BACKGROUND
BACKGROUND
The evolution of pathogenic mechanisms is a major challenge, which requires a thorough comprehension of the phylogenetic relationships of pathogens. Peronosporaleans encompasses a heterogeneous group of oomycetes that includes some animal/human pathogens, like Pythium insidiosum.
OBJECTIVE
OBJECTIVE
We analysed here the phylogenetic positioning and other evolutionary aspects related to this species and other peronosporaleans, using a multi-locus approach with one mitochondrial and three nuclear genes.
METHODOLOGY
METHODS
Phylogenetic patterns of 55 oomycetes were inferred by maximum likelihood and Bayesian analysis, and a relaxed molecular clock method was applied to infer the divergence time of some peronosporaleans branches.
RESULTS
RESULTS
Pythium insidiosum was monophyletic with a major and polytomous clade of American isolates; however, Pythium spp. was found to be paraphyletic with Phytopythium sp. and Phytophthora spp. In general, peronosporaleans subdivided into four lineages, one of which evidenced a close relationship of P insidiosum, P aphanidermatum and P arrhenomanes. This lineage diverged about 63 million years ago (Mya), whereas P insidiosum diversified at approximately 24 Mya. The divergence of American and Thai isolates seems to have occurred at approximately 17 Mya, with further American diversification at 2.4 Mya.
CONCLUSION
CONCLUSIONS
Overall, this study clarifies the phylogenetic relationships of P insidiosum regarding other peronosporaleans in a multi-locus perspective, despite previous claims that phylogenomic analyses are needed to accurately infer the patterns and processes related to the evolution of different lineages in this group. Additionally, this is the first time that a molecular clock was applied to study the evolution of P insidiosum.
Substances chimiques
DNA, Ribosomal Spacer
0
RNA, Ribosomal
0
Electron Transport Complex IV
EC 1.9.3.1
Banques de données
GENBANK
['JX115078', 'MH823870', 'MK000403', 'MF767408', 'MK000415', 'JN159478', 'KU714523', 'JN126280', 'MK000416', 'JN159479', 'MK000404', 'JN126281', 'MK053592', 'JN159477', 'KU739075', 'JN126282', 'MK000417', 'JN159480', 'KU739076', 'JN126283', 'MK000418', 'JN159483', 'MK053593', 'JN126286', 'MK000419', 'MH823868', 'KU739084', 'MH813295', 'MK000420', 'MH823869', 'MK000405', 'MH813296', 'MK000421', 'MH823871', 'MK000406', 'MH813297', 'MK000422', 'JN159487', 'KU739078', 'JN126290', 'MK000423', 'MH878801', 'MK000407', 'MH813298', 'MK000424', 'JN159490', 'MK000408', 'JN126293', 'MK000425', 'JN159492', 'KU739079', 'JN126295', 'MK000426', 'JN159493', 'KU739080', 'JN126296', 'MK000427', 'JN159495', 'KU739081', 'JN126298', 'MK000428', 'JN159496', 'KU739082', 'JN126299', 'MK000429', 'JN159497', 'KU739083', 'JN126300', 'MK000430', 'JN159499', 'KU840806', 'JN126302', 'MK000431', 'JN159501', 'MK000409', 'JN126304', 'MK000432', 'MH878800', 'MK000410', 'MH813300', 'MK000433', 'JN159503', 'MK000411', 'JN126306', 'MK000434', 'JN159504', 'MK000412', 'JN126307', 'MK000435', 'MH878799', 'KU840807', 'KJ176713', 'MK015630', 'MH878798', 'KU840808', 'MH813302', 'MK015631', 'MH878797', 'KU840809', 'MH813303', 'MK015632', 'MK000397', 'KU840810', 'MH813304', 'MK015633', 'MK000398', 'MK000413', 'MH813305', 'MK015634', 'MK000399', 'MK000414', 'MH813306', 'MK015635', 'MK000400', 'MK015629', 'AB898106', 'MK015636', 'MK000401', 'MK015627', 'AB971178', 'MK015637', 'MK000402', 'MK015628', 'AB971185', 'MK015638', 'JN159507', 'KU840811', 'JN126310', 'MK015639', 'KJ595391', 'AY598637']
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
395-406Subventions
Organisme : Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
ID : 001
Organisme : Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
ID : 420538/2018-6
Organisme : Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul
ID : PqG/FAPERGS 27293.414.15435.20062017
Informations de copyright
© 2020 Blackwell Verlag GmbH.
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