Maternal Overweight Downregulates MME (Neprilysin) in Feto-Placental Endothelial Cells and in Cord Blood.
MME
feto-placental endothelial cells
maternal overweight
neprilysin
umbilical cord blood
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
28 Jan 2020
28 Jan 2020
Historique:
received:
09
12
2019
revised:
21
01
2020
accepted:
23
01
2020
entrez:
5
2
2020
pubmed:
6
2
2020
medline:
31
10
2020
Statut:
epublish
Résumé
Maternal overweight in pregnancy alters the metabolic environment and generates chronic low-grade inflammation. This affects fetal development and programs the offspring's health for developing cardiovascular and metabolic disease later in life. MME (membrane-metalloendopeptidase, neprilysin) cleaves various peptides regulating vascular tone. Endothelial cells express membrane-bound and soluble MME. In adults, the metabolic environment of overweight and obesity upregulates endothelial and circulating MME. We here hypothesized that maternal overweight increases MME in the feto-placental endothelium. We used primary feto-placental endothelial cells (fpEC) isolated from placentas after normal vs. overweight pregnancies and determined MME mRNA, protein, and release. Additionally, soluble cord blood MME was analyzed. The effect of oxygen and tumor necrosis factor α (TNFα) on MME protein in fpEC was investigated in vitro. Maternal overweight reduced MME mRNA (-39.9%,
Identifiants
pubmed: 32012940
pii: ijms21030834
doi: 10.3390/ijms21030834
pmc: PMC7037888
pii:
doi:
Substances chimiques
Neprilysin
EC 3.4.24.11
Types de publication
Clinical Trial
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Austrian Science Fund FWF
ID : DOC 31
Pays : Austria
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