Altered productions of prostaglandins and prostamides by human amnion in response to infectious and inflammatory stimuli identified by mutliplex mass spectrometry.
Amnion
/ chemistry
Arachidonic Acid
/ chemistry
Arachidonic Acids
/ chemistry
Cytokines
/ adverse effects
Dinoprost
/ analogs & derivatives
Dinoprostone
/ analysis
Endocannabinoids
/ chemistry
Female
Humans
Interleukin-1beta
/ adverse effects
Lipopolysaccharides
/ adverse effects
Mass Spectrometry
Polyunsaturated Alkamides
/ chemistry
Pregnancy
Tumor Necrosis Factor-alpha
/ adverse effects
Amnion
Mass spectrometry
Prostaglandins
Prostamides
Journal
Prostaglandins, leukotrienes, and essential fatty acids
ISSN: 1532-2823
Titre abrégé: Prostaglandins Leukot Essent Fatty Acids
Pays: Scotland
ID NLM: 8802730
Informations de publication
Date de publication:
03 2020
03 2020
Historique:
received:
14
06
2019
revised:
28
11
2019
accepted:
21
01
2020
pubmed:
6
2
2020
medline:
22
9
2020
entrez:
5
2
2020
Statut:
ppublish
Résumé
Prostaglandins are critical for the onset and progression of labor in mammals, and are formed by the metabolism of arachidonic acid. The products of arachidonic acid, 2-arachidonoylglycerol (2-AG), and anandamide (AEA) have a similar lipid back bone but differing polar head groups, meaning that identification of these products by immunoassay can be difficult. In the current study, we present the use of mass spectrometry as multiplex method of identifying the specific end products of arachidonic and anandamide metabolism by human derived amnion explants treated with either an infectious agent (LPS) or inflammatory mediator (IL-1β or TNF-α). Human amnion tissue explants treated with LPS, IL-1β, or TNF-α increased production of prostaglandin E The findings of the current study are in keeping with the literature which describes amnion tissues as predominantly producing PGE
Identifiants
pubmed: 32014738
pii: S0952-3278(19)30123-1
doi: 10.1016/j.plefa.2020.102059
pii:
doi:
Substances chimiques
Arachidonic Acids
0
Cytokines
0
Endocannabinoids
0
Interleukin-1beta
0
Lipopolysaccharides
0
Polyunsaturated Alkamides
0
Tumor Necrosis Factor-alpha
0
15-keto-13,14-dihydroprostaglandin F2alpha
27376-76-7
Arachidonic Acid
27YG812J1I
Dinoprost
B7IN85G1HY
Dinoprostone
K7Q1JQR04M
anandamide
UR5G69TJKH
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
102059Informations de copyright
Copyright © 2020 Elsevier Ltd. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of competing interest None.