Inactivation of mesotrypsin by chymotrypsin C prevents trypsin inhibitor degradation.
chymotrypsin C
mesotrypsin
pancreas
protease
protease inhibitor
trypsin
trypsinogen
Journal
The Journal of biological chemistry
ISSN: 1083-351X
Titre abrégé: J Biol Chem
Pays: United States
ID NLM: 2985121R
Informations de publication
Date de publication:
13 03 2020
13 03 2020
Historique:
received:
03
01
2020
pubmed:
6
2
2020
medline:
15
12
2020
entrez:
5
2
2020
Statut:
ppublish
Résumé
Mesotrypsin is an unusual human trypsin isoform with inhibitor resistance and the ability to degrade trypsin inhibitors. Degradation of the protective serine protease inhibitor Kazal type 1 (SPINK1) by mesotrypsin in the pancreas may contribute to the pathogenesis of pancreatitis. Here we tested the hypothesis that the regulatory digestive protease chymotrypsin C (CTRC) mitigates the harmful effects of mesotrypsin by cleaving the autolysis loop. As human trypsins are post-translationally sulfated in the autolysis loop, we also assessed the effect of this modification. We found that mesotrypsin cleaved in the autolysis loop by CTRC exhibited catalytic impairment on short peptides due to a 10-fold increase in
Identifiants
pubmed: 32014997
pii: S0021-9258(17)48617-0
doi: 10.1074/jbc.RA120.012526
pmc: PMC7076226
pii:
doi:
Substances chimiques
Caseins
0
SPINK1 protein, human
0
Trypsin Inhibitors
0
Trypsin Inhibitor, Kazal Pancreatic
50936-63-5
Chymotrypsin
EC 3.4.21.1
chymotrypsin C
EC 3.4.21.2
PRSS3 protein, human
EC 3.4.21.4
Trypsin
EC 3.4.21.4
Banques de données
PDB
['2R9P']
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
3447-3455Subventions
Organisme : NIDDK NIH HHS
ID : R01 DK058088
Pays : United States
Organisme : NIDDK NIH HHS
ID : R01 DK082412
Pays : United States
Informations de copyright
© 2020 Toldi et al.
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