Evaluation of host-based molecular markers for the early detection of human sepsis.
Circulating nucleic acids
Host-based markers
Human sepsis diagnostics
Journal
Journal of biotechnology
ISSN: 1873-4863
Titre abrégé: J Biotechnol
Pays: Netherlands
ID NLM: 8411927
Informations de publication
Date de publication:
20 Feb 2020
20 Feb 2020
Historique:
received:
25
07
2019
revised:
18
01
2020
accepted:
27
01
2020
pubmed:
6
2
2020
medline:
8
10
2020
entrez:
5
2
2020
Statut:
ppublish
Résumé
We have identified 24 molecular markers, based on circulating nucleic acids (CNA) originating from the human genome, which in combination can be used in a quantitative real-time PCR (qPCR) assay to identify the presence of human sepsis, starting two to three days before the first clinical signs develop and including patients who meet the SEPSIS-3 criteria. The accuracy was more than 87 % inside of the same patient cohort for which the markers were developed and up to 81 % in blind studies of patient cohorts which were not included in the marker development. As our markers are host-based, they can be used to capture bacterial as well as fungal sepsis, unlike the current PCR-based tests, which require species-specific primer sets for each organism causing human sepsis. Our assay directly uses an aliquot of cell-free blood as the substrate for the PCR reaction, thus allowing to obtain the diagnostic results in three to four hours after the collection of the blood samples.
Identifiants
pubmed: 32017954
pii: S0168-1656(20)30015-8
doi: 10.1016/j.jbiotec.2020.01.013
pii:
doi:
Substances chimiques
Biomarkers
0
DNA, Bacterial
0
DNA, Fungal
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
80-88Subventions
Organisme : Austrian Science Fund FWF
ID : KLI 561
Pays : Austria
Informations de copyright
Copyright © 2020 Elsevier B.V. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Competing Interest None.