Multifaceted Analyses of Epidermal Serine Protease Activity in Patients with Atopic Dermatitis.
SPINK5
atopic dermatitis
epidermal barrier dysfunction
filaggrin
kallikrein-related peptidases
lympho-epithelial Kazal-type-related inhibitor (LEKTI)
serine proteases
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
30 Jan 2020
30 Jan 2020
Historique:
received:
18
12
2019
revised:
22
01
2020
accepted:
28
01
2020
entrez:
6
2
2020
pubmed:
6
2
2020
medline:
18
11
2020
Statut:
epublish
Résumé
The serine proteases kallikrein-related peptidase (KLK) 5 and KLK7 cleave cell adhesion molecules in the epidermis. Aberrant epidermal serine protease activity is thought to play an important role in the pathogenesis of atopic dermatitis (AD). We collected the stratum corneum (SC) from healthy individuals (n = 46) and AD patients (n = 63) by tape stripping and then measuring the trypsin- and chymotrypsin-like serine protease activity. We also analyzed the p.D386N and p.E420K of SPINK5 variants and loss-of-function mutations of FLG in the AD patients. The serine protease activity in the SC was increased not only in AD lesions but also in non-lesions of AD patients. We found, generally, that there was a positive correlation between the serine protease activity in the SC and the total serum immunoglobulin E (IgE) levels, serum thymus and activation-regulated chemokine (TARC) levels, and peripheral blood eosinophil counts. Moreover, the p.D386N or p.E420K in SPINK5 and FLG mutations were not significantly associated with the SC's serine protease activity. Epidermal serine protease activity was increased even in non-lesions of AD patients. Such activity was found to correlate with a number of biomarkers of AD. Further investigations of serine proteases might provide new treatments and prophylaxis for AD.
Identifiants
pubmed: 32019242
pii: ijms21030913
doi: 10.3390/ijms21030913
pmc: PMC7038095
pii:
doi:
Substances chimiques
Biomarkers
0
FLG protein, human
0
Filaggrin Proteins
0
S100 Proteins
0
SPINK5 protein, human
0
Serine Peptidase Inhibitor Kazal-Type 5
0
Serine Proteases
EC 3.4.-
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Grant-in-Aid for Scientific Research (C)
ID : 26461658
Déclaration de conflit d'intérêts
The authors declare no conflict of interest.
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