Three-Dimensional Visualization of APEX2-Tagged Erg11 in Saccharomyces cerevisiae Using Focused Ion Beam Scanning Electron Microscopy.
Cell Wall
/ ultrastructure
Cytochrome P-450 Enzyme System
/ ultrastructure
Endoplasmic Reticulum
/ ultrastructure
Imaging, Three-Dimensional
/ methods
Microscopy, Electron, Scanning
Saccharomyces cerevisiae
/ physiology
Saccharomyces cerevisiae Proteins
/ ultrastructure
Spheroplasts
/ ultrastructure
APEX2
DAB
FIB-SEM
Saccharomyces cerevisiae
electron microscopy
protein localization
spheroplast
yeast
Journal
mSphere
ISSN: 2379-5042
Titre abrégé: mSphere
Pays: United States
ID NLM: 101674533
Informations de publication
Date de publication:
05 02 2020
05 02 2020
Historique:
entrez:
7
2
2020
pubmed:
7
2
2020
medline:
21
10
2020
Statut:
epublish
Résumé
The determination of the exact location of a protein in the cell is essential to the understanding of biological processes. Here, we report for the first time the visualization of a protein of interest in
Identifiants
pubmed: 32024705
pii: 5/1/e00981-19
doi: 10.1128/mSphere.00981-19
pmc: PMC7002314
pii:
doi:
Substances chimiques
Saccharomyces cerevisiae Proteins
0
Cytochrome P-450 Enzyme System
9035-51-2
Erg11 protein, S cerevisiae
EC 1.14.14.1
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
Copyright © 2020 Kerstens et al.
Références
J Bacteriol. 1980 May;142(2):414-23
pubmed: 6991473
J Microsc. 2014 Jun;254(3):109-14
pubmed: 24707797
J Cell Sci. 2014 Sep 15;127(Pt 18):4078-88
pubmed: 25052096
Biotechniques. 2012 Jul;53(1):41-8
pubmed: 22780318
Nat Biotechnol. 2012 Nov;30(11):1143-8
pubmed: 23086203
J Biol Chem. 1992 Jan 25;267(3):2046-56
pubmed: 1730736
Micron. 2014 Jun;61:9-19
pubmed: 24792442
mBio. 2017 Jul 18;8(4):
pubmed: 28720726
Nucleic Acids Res. 1998 Aug 1;26(15):3577-83
pubmed: 9671822
Nat Methods. 2015 Jan;12(1):51-4
pubmed: 25419960
Dev Cell. 2015 Nov 23;35(4):513-25
pubmed: 26585296
Proc Natl Acad Sci U S A. 2014 Mar 11;111(10):3865-70
pubmed: 24613931
Biophys Rep. 2017;3(4):92-99
pubmed: 29238746
Lipids. 1993 Nov;28(11):963-7
pubmed: 8277826
Philos Trans R Soc Lond B Biol Sci. 2013 Jan 06;368(1612):20120434
pubmed: 23297356
Cold Spring Harb Perspect Med. 2014 May 01;4(5):
pubmed: 24789878
Drug Metab Rev. 1999 May;31(2):393-410
pubmed: 10335443
Biochem J. 2016 Aug 15;473(16):2463-9
pubmed: 27274088
Methods Cell Biol. 2001;65:37-51
pubmed: 11381604
Biochim Biophys Acta. 2005 Jul 15;1735(2):111-8
pubmed: 15951236
J Microsc. 2015 Aug;259(2):80-96
pubmed: 25623622
Genome Biol. 2000;1(6):REVIEWS3003
pubmed: 11178272
Yeast. 1995 May;11(6):493-536
pubmed: 7645343
Clin Microbiol Rev. 1998 Apr;11(2):382-402
pubmed: 9564569