Identification and characterization of novel filament-forming proteins in cyanobacteria.
Amino Acid Motifs
/ genetics
Anabaena
/ cytology
Bacterial Proteins
/ genetics
Cyanobacteria
/ cytology
Cytoskeletal Proteins
/ genetics
Cytoskeleton
/ metabolism
Genes, Bacterial
/ genetics
Mutation
Protein Conformation, alpha-Helical
/ genetics
Protein Multimerization
Recombinant Proteins
/ genetics
Synechococcus
/ cytology
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
05 02 2020
05 02 2020
Historique:
received:
28
08
2019
accepted:
17
01
2020
entrez:
7
2
2020
pubmed:
7
2
2020
medline:
21
11
2020
Statut:
epublish
Résumé
Filament-forming proteins in bacteria function in stabilization and localization of proteinaceous complexes and replicons; hence they are instrumental for myriad cellular processes such as cell division and growth. Here we present two novel filament-forming proteins in cyanobacteria. Surveying cyanobacterial genomes for coiled-coil-rich proteins (CCRPs) that are predicted as putative filament-forming proteins, we observed a higher proportion of CCRPs in filamentous cyanobacteria in comparison to unicellular cyanobacteria. Using our predictions, we identified nine protein families with putative intermediate filament (IF) properties. Polymerization assays revealed four proteins that formed polymers in vitro and three proteins that formed polymers in vivo. Fm7001 from Fischerella muscicola PCC 7414 polymerized in vitro and formed filaments in vivo in several organisms. Additionally, we identified a tetratricopeptide repeat protein - All4981 - in Anabaena sp. PCC 7120 that polymerized into filaments in vitro and in vivo. All4981 interacts with known cytoskeletal proteins and is indispensable for Anabaena viability. Although it did not form filaments in vitro, Syc2039 from Synechococcus elongatus PCC 7942 assembled into filaments in vivo and a Δsyc2039 mutant was characterized by an impaired cytokinesis. Our results expand the repertoire of known prokaryotic filament-forming CCRPs and demonstrate that cyanobacterial CCRPs are involved in cell morphology, motility, cytokinesis and colony integrity.
Identifiants
pubmed: 32024928
doi: 10.1038/s41598-020-58726-9
pii: 10.1038/s41598-020-58726-9
pmc: PMC7002697
doi:
Substances chimiques
Bacterial Proteins
0
Cytoskeletal Proteins
0
Recombinant Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1894Références
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