GPRASP proteins are critical negative regulators of hematopoietic stem cell transplantation.
Animals
Carrier Proteins
Cell Movement
Cell Proliferation
Cell Survival
Gene Deletion
Gene Silencing
Hematopoietic Stem Cell Transplantation
/ methods
Hematopoietic Stem Cells
/ cytology
Humans
Intracellular Signaling Peptides and Proteins
/ genetics
Mice
Mice, Inbred C57BL
Proteolysis
Receptors, CXCR4
/ metabolism
Receptors, G-Protein-Coupled
/ genetics
Journal
Blood
ISSN: 1528-0020
Titre abrégé: Blood
Pays: United States
ID NLM: 7603509
Informations de publication
Date de publication:
02 04 2020
02 04 2020
Historique:
received:
20
09
2019
accepted:
21
01
2020
pubmed:
7
2
2020
medline:
3
11
2020
entrez:
7
2
2020
Statut:
ppublish
Résumé
Hematopoietic stem cell (HSC) transplantation (HSCT) is often exploited to treat hematologic disease. Donor HSCs must survive, proliferate, and differentiate in the damaged environment of the reconstituting niche. Illuminating molecular mechanisms regulating the activity of transplanted HSCs will inform efforts to improve HSCT. Here, we report that G-protein-coupled receptor-associated sorting proteins (GPRASPs) function as negative regulators of HSCT. Silencing of Gprasp1 or Gprasp2 increased the survival, quiescence, migration, niche retention, and hematopoietic repopulating activity of hematopoietic stem and progenitor cells (HSPCs) posttransplant. We further show that GPRASP1 and GPRASP2 promote the degradation of CXCR4, a master regulator of HSC function during transplantation. CXCR4 accumulates in Gprasp-deficient HSPCs, boosting their function posttransplant. Thus, GPRASPs negatively regulate CXCR4 stability in HSCs. Our work reveals GPRASP proteins as negative regulators of HSCT and CXCR4 activity. Disruption of GPRASP/CXCR4 interactions could be exploited in the future to enhance the efficiency of HSCT.
Identifiants
pubmed: 32027737
pii: S0006-4971(20)62134-6
doi: 10.1182/blood.2019003435
pmc: PMC7118811
doi:
Substances chimiques
CXCR4 protein, mouse
0
Carrier Proteins
0
Gprasp1 protein, mouse
0
Gprasp2 protein, mouse
0
Intracellular Signaling Peptides and Proteins
0
Receptors, CXCR4
0
Receptors, G-Protein-Coupled
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1111-1123Commentaires et corrections
Type : CommentIn
Informations de copyright
© 2020 by The American Society of Hematology.
Références
Blood. 2001 May 15;97(10):2962-71
pubmed: 11342418
Cell Stem Cell. 2011 Jan 7;8(1):72-83
pubmed: 21211783
Contrib Microbiol. 2006;13:191-199
pubmed: 16627966
Biochim Biophys Acta. 2007 Apr;1768(4):952-63
pubmed: 17169327
Blood. 2002 Feb 15;99(4):1117-29
pubmed: 11830456
J Neurochem. 2004 May;89(3):766-75
pubmed: 15086532
Blood. 2016 Nov 24;128(21):2561-2567
pubmed: 27625358
Dev Dyn. 2005 May;233(1):188-93
pubmed: 15759267
J Exp Med. 2008 Apr 14;205(4):777-83
pubmed: 18378795
Stem Cells Dev. 2013 Dec;22 Suppl 1:103-10
pubmed: 24304086
Biol Blood Marrow Transplant. 1996 May;2(2):100-4
pubmed: 9118298
J Leukoc Biol. 2003 May;73(5):630-8
pubmed: 12714578
Blood. 2011 Jan 13;117(2):429-39
pubmed: 20833981
Eur J Med Genet. 2016 May;59(5):274-7
pubmed: 26995686
Biol Blood Marrow Transplant. 2018 Aug;24(8):1759-1765
pubmed: 29656137
Mol Cell. 2004 Sep 24;15(6):853-65
pubmed: 15383276
Proc Natl Acad Sci U S A. 1999 May 11;96(10):5663-7
pubmed: 10318941
J Bone Miner Res. 2011 Apr;26(4):747-60
pubmed: 20954177
J Biol Chem. 2004 Dec 24;279(52):54291-303
pubmed: 15452121
J Immunol. 2003 Jan 1;170(1):421-9
pubmed: 12496427
Curr Opin Mol Ther. 2003 Oct;5(5):508-16
pubmed: 14601520
Pharmacol Ther. 2010 Jun;126(3):244-50
pubmed: 20394773
Best Pract Res Clin Haematol. 2018 Dec;31(4):410-413
pubmed: 30466758
Biol Blood Marrow Transplant. 2010 Jan;16(1 Suppl):S126-32
pubmed: 19896543
Am J Pediatr Hematol Oncol. 1994 Feb;16(1):27-9
pubmed: 8311169
J Biol Chem. 2009 May 15;284(20):13629-40
pubmed: 19304657
Acta Med Okayama. 2019 Apr;73(2):161-171
pubmed: 31015751
Blood. 2005 Sep 15;106(6):1901-10
pubmed: 15890683
N Engl J Med. 2004 Nov 25;351(22):2265-75
pubmed: 15564543
Crit Rev Oncol Hematol. 2019 Jan;133:120-128
pubmed: 30661648
Mol Pharmacol. 2007 Feb;71(2):494-507
pubmed: 17110500
J Cell Biochem. 2004 Apr 1;91(5):1030-42
pubmed: 15034937
Front Cell Neurosci. 2016 Mar 03;10:47
pubmed: 26973462
Blood. 2006 Mar 15;107(6):2243-51
pubmed: 16291599
Blood. 1997 Jun 1;89(11):4013-20
pubmed: 9166840
Curr Biol. 2007 Jul 3;17(13):1164-72
pubmed: 17600713
Antioxid Redox Signal. 2014 Oct 10;21(11):1605-19
pubmed: 24762207
J Neurosci. 2007 Apr 11;27(15):4165-77
pubmed: 17428994
Blood. 2006 Mar 1;107(5):1761-7
pubmed: 16269611
J Exp Med. 2016 Mar 7;213(3):433-49
pubmed: 26880577
PLoS One. 2013;8(2):e56336
pubmed: 23441177
Science. 1999 Feb 5;283(5403):845-8
pubmed: 9933168
Biol Blood Marrow Transplant. 2009 Jun;15(6):662-70
pubmed: 19450750
Nat Chem Biol. 2010 Mar;6(3):209-217
pubmed: 20118940
Hum Mol Genet. 2014 Jul 15;23(14):3823-9
pubmed: 24569167
J Cell Mol Med. 2011 Nov;15(11):2462-77
pubmed: 21199326
Pharmacology. 2010;86(1):22-9
pubmed: 20693822
Nature. 1998 Jun 11;393(6685):595-9
pubmed: 9634238
Oncogene. 2004 Sep 20;23(43):7223-32
pubmed: 15378082
Nature. 1996 Aug 15;382(6592):635-8
pubmed: 8757135
J Biol Chem. 2007 Oct 5;282(40):29178-85
pubmed: 17635908
Stem Cells. 2007 Feb;25(2):279-88
pubmed: 17008427
Exp Biol Med (Maywood). 2011 Dec;236(12):1413-9
pubmed: 22101518
Science. 2002 Jul 26;297(5581):615-20
pubmed: 12142540
Immunol Rev. 2000 Oct;177:175-84
pubmed: 11138774
J Med Case Rep. 2017 Aug 17;11(1):226
pubmed: 28814329
Exp Hematol. 2006 Nov;34(11):1553-62
pubmed: 17046575
Transplant Proc. 2019 Apr;51(3):890-895
pubmed: 30979481
Blood. 2012 Nov 1;120(18):3677-87
pubmed: 22833548
N Engl J Med. 2014 Jul 24;371(4):339-48
pubmed: 25054717
World J Oncol. 2019 Feb;10(1):1-9
pubmed: 30834047
Blood. 2004 Apr 15;103(8):2942-9
pubmed: 15070669
J Exp Med. 1997 Jan 6;185(1):111-20
pubmed: 8996247
FASEB J. 2007 Mar;21(3):802-11
pubmed: 17197383
PLoS One. 2016 Feb 22;11(2):e0149477
pubmed: 26901434
Nat Rev Drug Discov. 2008 Apr;7(4):339-57
pubmed: 18382464
Blood. 1998 Jun 15;91(12):4523-30
pubmed: 9616148
Proc Natl Acad Sci U S A. 2005 Aug 9;102(32):11521-6
pubmed: 16049099
J Cell Biol. 2010 Jul 26;190(2):165-75
pubmed: 20643879
Biochemistry. 2012 Jul 31;51(30):5951-7
pubmed: 22779681
J Biol Chem. 2011 Jan 14;286(2):1598-608
pubmed: 21030592
N Engl J Med. 2004 Nov 25;351(22):2276-85
pubmed: 15564544
Biophys J. 2010 Nov 3;99(9):3102-11
pubmed: 21044609
Lancet. 2007 Jun 9;369(9577):1947-54
pubmed: 17560447
Neuron. 2016 Dec 21;92(6):1294-1307
pubmed: 28009275
Immunity. 2006 Dec;25(6):977-88
pubmed: 17174120
Sci Rep. 2016 Mar 02;6:22540
pubmed: 26931153