Curcumin Derivative GT863 Inhibits Amyloid-Beta Production via Inhibition of Protein N-Glycosylation.


Journal

Cells
ISSN: 2073-4409
Titre abrégé: Cells
Pays: Switzerland
ID NLM: 101600052

Informations de publication

Date de publication:
03 02 2020
Historique:
received: 27 12 2019
revised: 29 01 2020
accepted: 31 01 2020
entrez: 8 2 2020
pubmed: 8 2 2020
medline: 13 2 2021
Statut: epublish

Résumé

Amyloid-β (Aβ) peptides play a crucial role in the pathogenesis of Alzheimer's disease (AD). Aβ production, aggregation, and clearance are thought to be important therapeutic targets for AD. Curcumin has been known to have an anti-amyloidogenic effect on AD. In the present study, we performed screening analysis using a curcumin derivative library with the aim of finding derivatives effective in suppressing Aβ production with improved bioavailability of curcumin using CHO cells that stably express human amyloid-β precursor protein and using human neuroblastoma SH-SY5Y cells. We found that the curcumin derivative GT863/PE859, which has been shown to have an inhibitory effect on Aβ and tau aggregation in vivo, was more effective than curcumin itself in reducing Aβ secretion. We further found that GT863 inhibited neither β- nor γ-secretase activity, but did suppress γ-secretase-mediated cleavage in a substrate-dependent manner. We further found that GT863 suppressed

Identifiants

pubmed: 32028683
pii: cells9020349
doi: 10.3390/cells9020349
pmc: PMC7072163
pii:
doi:

Substances chimiques

Alkaloids 0
Amyloid beta-Peptides 0
Membrane Glycoproteins 0
Receptors, Notch 0
nicastrin protein 0
kifunensine 0NI8960271
Mannosidases EC 3.2.1.-
Amyloid Precursor Protein Secretases EC 3.4.-
Curcumin IT942ZTH98
Swainsonine RSY4RK37KQ

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Déclaration de conflit d'intérêts

H.S. is President of Green Tech Co., Ltd. Other authors declare no conflict of interest.

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Auteurs

Yasuomi Urano (Y)

Department of Medical Life Systems, Faculty of Life and Medical Sciences, Doshisha University, 1-3 Miyakodani, Tatara, Kyotanabe, Kyoto 610 0394, Japan.

Mina Takahachi (M)

Department of Medical Life Systems, Faculty of Life and Medical Sciences, Doshisha University, 1-3 Miyakodani, Tatara, Kyotanabe, Kyoto 610 0394, Japan.

Ryo Higashiura (R)

Department of Medical Life Systems, Faculty of Life and Medical Sciences, Doshisha University, 1-3 Miyakodani, Tatara, Kyotanabe, Kyoto 610 0394, Japan.

Hitomi Fujiwara (H)

Department of Medical Life Systems, Faculty of Life and Medical Sciences, Doshisha University, 1-3 Miyakodani, Tatara, Kyotanabe, Kyoto 610 0394, Japan.

Satoru Funamoto (S)

Department of Medical Life Systems, Faculty of Life and Medical Sciences, Doshisha University, 1-3 Miyakodani, Tatara, Kyotanabe, Kyoto 610 0394, Japan.

So Imai (S)

Department of Molecular and Cell Biology, Graduate School of Agricultural Science, Tohoku University, 468-1 Aramakiazaaoba, Aobaku, Sendai, Miyagi 980-0845, Japan.

Eugene Futai (E)

Department of Molecular and Cell Biology, Graduate School of Agricultural Science, Tohoku University, 468-1 Aramakiazaaoba, Aobaku, Sendai, Miyagi 980-0845, Japan.

Michiaki Okuda (M)

Green Tech Co., Ltd., 1-7-7 Yaesu, Chuo-ku, Tokyo 103-0028, Japan.

Hachiro Sugimoto (H)

Department of Medical Life Systems, Faculty of Life and Medical Sciences, Doshisha University, 1-3 Miyakodani, Tatara, Kyotanabe, Kyoto 610 0394, Japan.
Green Tech Co., Ltd., 1-7-7 Yaesu, Chuo-ku, Tokyo 103-0028, Japan.

Noriko Noguchi (N)

Department of Medical Life Systems, Faculty of Life and Medical Sciences, Doshisha University, 1-3 Miyakodani, Tatara, Kyotanabe, Kyoto 610 0394, Japan.

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Classifications MeSH