Structure of the secretory immunoglobulin A core.


Journal

Science (New York, N.Y.)
ISSN: 1095-9203
Titre abrégé: Science
Pays: United States
ID NLM: 0404511

Informations de publication

Date de publication:
28 02 2020
Historique:
received: 20 09 2019
accepted: 24 01 2020
pubmed: 8 2 2020
medline: 6 5 2020
entrez: 8 2 2020
Statut: ppublish

Résumé

Secretory immunoglobulin A (sIgA) represents the immune system's first line of defense against mucosal pathogens. IgAs are transported across the epithelium, as dimers and higher-order polymers, by the polymeric immunoglobulin receptor (pIgR). Upon reaching the luminal side, sIgAs mediate host protection and pathogen neutralization. In recent years, an increasing amount of attention has been given to IgA as a novel therapeutic antibody. However, despite extensive studies, sIgA structures have remained elusive. Here, we determine the atomic resolution structures of dimeric, tetrameric, and pentameric IgA-Fc linked by the joining chain (JC) and in complex with the secretory component of the pIgR. We suggest a mechanism in which the JC templates IgA oligomerization and imparts asymmetry for pIgR binding and transcytosis. This framework will inform the design of future IgA-based therapeutics.

Identifiants

pubmed: 32029686
pii: science.aaz5807
doi: 10.1126/science.aaz5807
doi:

Substances chimiques

Immunoglobulin A, Secretory 0
Immunoglobulin Fc Fragments 0
Immunoglobulin J-Chains 0
Receptors, Polymeric Immunoglobulin 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

1008-1014

Informations de copyright

Copyright © 2020 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works.

Auteurs

Nikit Kumar (N)

Department of Structural Biology, Genentech, Inc., South San Francisco, CA, USA 94080.

Christopher P Arthur (CP)

Department of Structural Biology, Genentech, Inc., South San Francisco, CA, USA 94080.

Claudio Ciferri (C)

Department of Structural Biology, Genentech, Inc., South San Francisco, CA, USA 94080. ciferri.claudio@gene.com matsumoto.marissa@gene.com.

Marissa L Matsumoto (ML)

Department of Structural Biology, Genentech, Inc., South San Francisco, CA, USA 94080. ciferri.claudio@gene.com matsumoto.marissa@gene.com.

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Classifications MeSH