Biochemical control of Alternaria tenuissima infecting post-harvest fig fruit by chickpea vicilin.
Alternaria tenuissima
antifungal
fig fruit
postharvest
scanning electron microscopy
vicilin
Journal
Journal of the science of food and agriculture
ISSN: 1097-0010
Titre abrégé: J Sci Food Agric
Pays: England
ID NLM: 0376334
Informations de publication
Date de publication:
May 2020
May 2020
Historique:
received:
29
09
2019
revised:
22
12
2019
accepted:
07
02
2020
pubmed:
8
2
2020
medline:
11
11
2020
entrez:
8
2
2020
Statut:
ppublish
Résumé
Alternaria tenuissima was isolated from infected fig fruit and molecularly identified by rRNA gene sequencing. The objective of the current work was to test the inhibitory effect of vicilin as a glycoprotein, isolated from chickpea, against the fungus A. tenuissima, isolated from fig fruit, in vitro and in situ, to estimate its potential action in controlling the growth of A. tenuissima in postharvest fig fruit. Chickpea vicilin is a glycoprotein composed of three subunits of 135, 210, and 230 kDa. The linear growth of A. tenuissima on the solid agar medium and in liquid media (at 25 °C) was markedly reduced by 44%, 66%, 77%, and 83% and 20%, 24%, 42%, and 62%, respectively in response to vicilin applications of 0.1, 0.2, 0.3, and 0.4 g L Vicilin can be considered a potent antifungal agent that can be used in preserving fig fruit for 7-14 days with minimum disease severity. © 2020 Society of Chemical Industry.
Sections du résumé
BACKGROUND
BACKGROUND
Alternaria tenuissima was isolated from infected fig fruit and molecularly identified by rRNA gene sequencing. The objective of the current work was to test the inhibitory effect of vicilin as a glycoprotein, isolated from chickpea, against the fungus A. tenuissima, isolated from fig fruit, in vitro and in situ, to estimate its potential action in controlling the growth of A. tenuissima in postharvest fig fruit.
RESULTS
RESULTS
Chickpea vicilin is a glycoprotein composed of three subunits of 135, 210, and 230 kDa. The linear growth of A. tenuissima on the solid agar medium and in liquid media (at 25 °C) was markedly reduced by 44%, 66%, 77%, and 83% and 20%, 24%, 42%, and 62%, respectively in response to vicilin applications of 0.1, 0.2, 0.3, and 0.4 g L
CONCLUSION
CONCLUSIONS
Vicilin can be considered a potent antifungal agent that can be used in preserving fig fruit for 7-14 days with minimum disease severity. © 2020 Society of Chemical Industry.
Substances chimiques
Fungicides, Industrial
0
Seed Storage Proteins
0
vicilin protein, plant
9067-60-1
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
2889-2897Subventions
Organisme : Zagazig University
Informations de copyright
© 2020 Society of Chemical Industry.
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