Limited diagnostic possibilities for bloodstream infections with broad-range methods: A promising PCR/electrospray ionization-mass spectrometry platform is no longer available.
Adult
Aged
Aged, 80 and over
Bacteremia
/ diagnosis
Female
Humans
Male
Microbiological Techniques
Middle Aged
Molecular Diagnostic Techniques
/ methods
Polymerase Chain Reaction
/ methods
RNA, Ribosomal, 16S
Reproducibility of Results
Sepsis
/ diagnosis
Spectrometry, Mass, Electrospray Ionization
/ methods
Young Adult
16S PCR
PCR/ESI-MS
bloodstream infections
broad-range PCR
molecular diagnostics
Journal
MicrobiologyOpen
ISSN: 2045-8827
Titre abrégé: Microbiologyopen
Pays: England
ID NLM: 101588314
Informations de publication
Date de publication:
05 2020
05 2020
Historique:
received:
25
11
2019
revised:
15
01
2020
accepted:
16
01
2020
pubmed:
8
2
2020
medline:
30
4
2021
entrez:
8
2
2020
Statut:
ppublish
Résumé
Fast and accurate detection of causative agents of bloodstream infections remains a challenge of today's microbiology. We compared the performance of cutting-edge technology based on polymerase chain reaction coupled with electrospray ionization-mass spectrometry (PCR/ESI-MS) with that of conventional broad-range 16S rRNA PCR and blood culture to address the current diagnostic possibilities for bloodstream infections. Of 160 blood samples tested, PCR/ESI-MS revealed clinically meaningful microbiological agents in 47 samples that were missed by conventional diagnostic approaches (29.4% of all analyzed samples). Notably, PCR/ESI-MS shortened the time to positivity of the blood culture-positive samples by an average of 34 hr. PCR/ESI-MS technology substantially improved current diagnostic tools and represented an opportunity to make bloodstream infections diagnostics sensitive, accurate, and timely with a broad spectrum of microorganisms covered.
Identifiants
pubmed: 32031761
doi: 10.1002/mbo3.1007
pmc: PMC7221429
doi:
Substances chimiques
RNA, Ribosomal, 16S
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e1007Informations de copyright
© 2020 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd.
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