A Truncated Galectin-3 Isolated from Skin Mucus of Atlantic Salmon
Gram-negative
agglutination
bacteria
galectin-3
hemagglutination
innate immunology
lectin
mucosal immunology
multidrug transporter
proteomics
Journal
Marine drugs
ISSN: 1660-3397
Titre abrégé: Mar Drugs
Pays: Switzerland
ID NLM: 101213729
Informations de publication
Date de publication:
04 Feb 2020
04 Feb 2020
Historique:
received:
22
12
2019
revised:
31
01
2020
accepted:
02
02
2020
entrez:
9
2
2020
pubmed:
9
2
2020
medline:
2
7
2020
Statut:
epublish
Résumé
The mucus of fish skin plays a vital role in innate immune defense. Some mucus proteins have the potential to incapacitate pathogens and/or inhibit their passage through the skin. In this study the aim was to isolate and characterize galectin(s), β-galactosides binding proteins, present in skin mucus. A novel short form of galectin-3 was isolated from Atlantic salmon skin mucus by α-lactose agarose based affinity chromatography followed by Sephadex G-15 gel filtration. Mass spectrometric analysis showed that the isolated protein was the C-terminal half of galectin-3 (galectin-3C). Galectin-3C showed calcium independent and lactose inhabitable hemagglutination, and agglutinated the Gram-negative pathogenic bacteria
Identifiants
pubmed: 32033203
pii: md18020102
doi: 10.3390/md18020102
pmc: PMC7074318
pii:
doi:
Substances chimiques
Carrier Proteins
0
Fish Proteins
0
Galectin 3
0
Peptides
0
Proteome
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Déclaration de conflit d'intérêts
The authors declare no conflict of interest.
Références
Antimicrob Agents Chemother. 2003 Mar;47(3):948-55
pubmed: 12604526
Nature. 1970 Aug 15;227(5259):680-5
pubmed: 5432063
Mol Immunol. 2006 Mar;43(8):1194-201
pubmed: 16139890
Clin Cancer Res. 2003 Jun;9(6):2374-83
pubmed: 12796408
Mil Med. 2017 Mar;182(S1):383-388
pubmed: 28291503
Exp Cell Res. 1993 Jul;207(1):8-18
pubmed: 8319774
Biomolecules. 2018 Sep 04;8(3):
pubmed: 30181497
IUBMB Life. 2013 Jul;65(7):633-44
pubmed: 23794513
Ann Transl Med. 2014 Sep;2(9):89
pubmed: 25405164
Methods Mol Biol. 2015;1207:431-49
pubmed: 25253157
Biochemistry. 1998 Mar 24;37(12):4086-92
pubmed: 9521730
Eur J Biochem. 1999 Sep;264(2):569-76
pubmed: 10491105
Biochem Biophys Res Commun. 2011 Jun 24;410(1):91-6
pubmed: 21640083
Fish Shellfish Immunol. 2011 Aug;31(2):224-31
pubmed: 21609766
Dev Comp Immunol. 2013 Jun;40(2):83-93
pubmed: 23416931
Int J Syst Evol Microbiol. 2000 Mar;50 Pt 2:427-450
pubmed: 10758846
Curr Protein Pept Sci. 2015;16(4):337-51
pubmed: 25929869
J Biol Chem. 1977 Sep 10;252(17):6026-30
pubmed: 408346
Nature. 2017 Apr 27;544(7651):498-502
pubmed: 28405025
J Antimicrob Chemother. 2018 Aug 1;73(8):2003-2020
pubmed: 29506149
Comp Biochem Physiol B Biochem Mol Biol. 2003 Dec;136(4):723-30
pubmed: 14662297
Autophagy. 2007 Jul-Aug;3(4):300-22
pubmed: 17377489
Mucosal Immunol. 2015 Jan;8(1):176-85
pubmed: 24962092
Dev Comp Immunol. 2004 Apr;28(4):325-35
pubmed: 14698218
BMC Mol Biol. 2005 Nov 17;6:21
pubmed: 16293192
Ann Clin Lab Sci. 1973 Mar-Apr;3(2):135-40
pubmed: 4575155
Molecules. 2018 May 09;23(5):
pubmed: 29747390
Protein Pept Lett. 2019 Nov 04;:
pubmed: 31682206
Biochemistry. 1994 Nov 29;33(47):14109-14
pubmed: 7947821
Mediators Inflamm. 2018 May 21;2018:9186940
pubmed: 29950926
Dev Comp Immunol. 2013 Jan-Feb;39(1-2):27-38
pubmed: 22561073
Nature. 2012 Jan 15;482(7385):414-8
pubmed: 22246324
mBio. 2018 Sep 18;9(5):
pubmed: 30228237
Nat Med. 2010 Mar;16(3):295-301
pubmed: 20154696
J Parasitol. 2000 Dec;86(6):1199-205
pubmed: 11191891
Mol Biosyst. 2016 Jun 21;12(7):2056-63
pubmed: 27173837
Int J Antimicrob Agents. 2009 Oct;34(4):355-9
pubmed: 19505801
Fish Shellfish Immunol. 2012 Oct;33(4):1008-15
pubmed: 22960031
J Biol Chem. 2004 Mar 19;279(12):10841-7
pubmed: 14672941
Microb Pathog. 2007 Jan;42(1):36-45
pubmed: 17145162
Trends Biochem Sci. 2018 Sep;43(9):668-684
pubmed: 30054232
Dev Comp Immunol. 1988 Spring;12(2):309-18
pubmed: 3384160
Mediators Inflamm. 2017;2017:9247574
pubmed: 28607536
Proc Natl Acad Sci U S A. 2018 May 8;115(19):E4396-E4405
pubmed: 29686075
Cell. 2000 Dec 22;103(7):1143-54
pubmed: 11163189
Environ Microbiol. 2018 Nov;20(11):4091-4111
pubmed: 30207068
Cell. 1991 Jan 11;64(1):91-7
pubmed: 1986871