Comparison of immune responses to Loa loa stage-specific antigen extracts in Loa loa-exposed BALB/c mice upon clearance of infection.
Animals
Antigens, Helminth
/ blood
Cytokines
/ blood
Diptera
/ parasitology
Humans
Immunity, Humoral
Immunoglobulins
/ blood
Insect Vectors
/ parasitology
Larva
/ parasitology
Life Cycle Stages
/ immunology
Loa
/ immunology
Loiasis
/ immunology
Mice
Mice, Inbred BALB C
/ immunology
Neglected Diseases
/ immunology
Th17 Cells
/ immunology
Th2 Cells
/ immunology
Adult worms
Chemokines
Cytokines
Immunoglobulins
Larvae
Loa loa antigen extract
Microfilariae
Re-stimulation
Recall immune responses
Journal
Parasites & vectors
ISSN: 1756-3305
Titre abrégé: Parasit Vectors
Pays: England
ID NLM: 101462774
Informations de publication
Date de publication:
07 Feb 2020
07 Feb 2020
Historique:
received:
20
08
2019
accepted:
31
01
2020
entrez:
9
2
2020
pubmed:
9
2
2020
medline:
6
6
2020
Statut:
epublish
Résumé
Different immune mechanisms are capable of killing developmental stages of filarial nematodes and these mechanisms are also likely to vary between the primary and a challenge infection. However, the lack of a detailed analysis of cytokine, chemokine and immunoglobulin levels in human loiasis is still evident. Therefore, detailed analysis of immune responses induced by the different developmental stages of Loa loa in immune-competent BALB/c mice will aid in the characterization of distinct immune responses that are important for the immunity against loiasis. Different developmental stages of L. loa were obtained from human peripheral blood (microfilariae, MF), the transmitting vector, Chrysops (larval stage 3, L3) and infected immune-deficient BALB/cRAG2γc In a pilot study, BALB/c mice cleared the different life stages of L. loa within 42 days pi and systemic cytokine, chemokine and immunoglobulin levels were equal between infected and naive mice. Nevertheless, L. loa-specific re-stimulation of splenocytes from mice infected with L5, MF or adult worms led to induction of Th2, Th17 and chemokine secretion patterns. This study shows that although host immunity remains comparable to naive mice, clearance of L. loa life-cycle development stages can induce immune cell memory leading to cytokine, chemokine and immunoglobulins secretion patterns which might contribute to immunity and protection against reinfection.
Sections du résumé
BACKGROUND
BACKGROUND
Different immune mechanisms are capable of killing developmental stages of filarial nematodes and these mechanisms are also likely to vary between the primary and a challenge infection. However, the lack of a detailed analysis of cytokine, chemokine and immunoglobulin levels in human loiasis is still evident. Therefore, detailed analysis of immune responses induced by the different developmental stages of Loa loa in immune-competent BALB/c mice will aid in the characterization of distinct immune responses that are important for the immunity against loiasis.
METHODS
METHODS
Different developmental stages of L. loa were obtained from human peripheral blood (microfilariae, MF), the transmitting vector, Chrysops (larval stage 3, L3) and infected immune-deficient BALB/cRAG2γc
RESULTS
RESULTS
In a pilot study, BALB/c mice cleared the different life stages of L. loa within 42 days pi and systemic cytokine, chemokine and immunoglobulin levels were equal between infected and naive mice. Nevertheless, L. loa-specific re-stimulation of splenocytes from mice infected with L5, MF or adult worms led to induction of Th2, Th17 and chemokine secretion patterns.
CONCLUSIONS
CONCLUSIONS
This study shows that although host immunity remains comparable to naive mice, clearance of L. loa life-cycle development stages can induce immune cell memory leading to cytokine, chemokine and immunoglobulins secretion patterns which might contribute to immunity and protection against reinfection.
Identifiants
pubmed: 32033624
doi: 10.1186/s13071-020-3921-x
pii: 10.1186/s13071-020-3921-x
pmc: PMC7006431
doi:
Substances chimiques
Antigens, Helminth
0
Cytokines
0
Immunoglobulins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
51Subventions
Organisme : Bundesministerium für Bildung und Forschung
ID : TAKeOFF
Organisme : Deutsches Zentrum für Infektionsforschung
ID : DZIF
Organisme : Deutsche Forschungsgemeinschaft
ID : EXC1023
Organisme : Deutsche Forschungsgemeinschaft
ID : RI 3036/1-1
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