High-resolution structural insights into the heliorhodopsin family.

Computational Biology Hydrogen-Ion Concentration Models, Molecular Photolysis Protein Conformation Rhodopsins, Microbial / chemistry
X-ray crystallography crystal structure membrane protein retinal rhodopsin

Journal

Proceedings of the National Academy of Sciences of the United States of America
ISSN: 1091-6490
Titre abrégé: Proc Natl Acad Sci U S A
Pays: United States
ID NLM: 7505876

Informations de publication

Date de publication:
25 02 2020
Historique:
pubmed: 9 2 2020
medline: 1 7 2020
entrez: 9 2 2020
Statut: ppublish

Résumé

Rhodopsins are the most abundant light-harvesting proteins. A new family of rhodopsins, heliorhodopsins (HeRs), has recently been discovered. Unlike in the known rhodopsins, in HeRs the N termini face the cytoplasm. The function of HeRs remains unknown. We present the structures of the bacterial HeR-48C12 in two states at the resolution of 1.5 Å, which highlight its remarkable difference from all known rhodopsins. The interior of HeR's extracellular part is completely hydrophobic, while the cytoplasmic part comprises a cavity (Schiff base cavity [SBC]) surrounded by charged amino acids and containing a cluster of water molecules, presumably being a primary proton acceptor from the Schiff base. At acidic pH, a planar triangular molecule (acetate) is present in the SBC. Structure-based bioinformatic analysis identified 10 subfamilies of HeRs, suggesting their diverse biological functions. The structures and available data suggest an enzymatic activity of HeR-48C12 subfamily and their possible involvement in fundamental redox biological processes.

Identifiants

DOI: 10.1073/pnas.1915888117 PMID: 32034096 PMC: PMC7049168
pubmed: 32034096
pii: 1915888117
doi: 10.1073/pnas.1915888117
pmc: PMC7049168
doi:

Substances chimiques

Rhodopsins, Microbial 0

Banques de données

PDB
['6SU3', '6SU4']

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

4131-4141

Commentaires et corrections

Type : ErratumIn

Informations de copyright

Copyright © 2020 the Author(s). Published by PNAS.

Déclaration de conflit d'intérêts

The authors declare no competing interest.

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Auteurs

K Kovalev (K)

Institut de Biologie Structurale J.-P. Ebel, Université Grenoble Alpes-Commission for Atomic Energy (CEA)-CNRS, 38000 Grenoble, France.
Institute of Biological Information Processing (Institute of Biological Information Processing: Structural Biochemistry), Forschungszentrum Jülich, 52428 Jülich, Germany.
JuStruct: Jülich Center for Structural Biology, Forschungszentrum Jülich, 52428 Jülich, Germany.
Research Center for Mechanisms of Aging and Age Related Diseases, Moscow Institute of Physics and Technology (National Research University), Dolgoprudny 141701, Russia.
Institute of Crystallography, University of Aachen (Rheinisch-Westfälische Technische Hochschule Aachen [RWTH]), 52062 Aachen, Germany.

D Volkov (D)

Institute of Biological Information Processing (Institute of Biological Information Processing: Structural Biochemistry), Forschungszentrum Jülich, 52428 Jülich, Germany.
JuStruct: Jülich Center for Structural Biology, Forschungszentrum Jülich, 52428 Jülich, Germany.
ORCID: 0000-0003-2451-0091

R Astashkin (R)

Institut de Biologie Structurale J.-P. Ebel, Université Grenoble Alpes-Commission for Atomic Energy (CEA)-CNRS, 38000 Grenoble, France.
Research Center for Mechanisms of Aging and Age Related Diseases, Moscow Institute of Physics and Technology (National Research University), Dolgoprudny 141701, Russia.

A Alekseev (A)

Institute of Biological Information Processing (Institute of Biological Information Processing: Structural Biochemistry), Forschungszentrum Jülich, 52428 Jülich, Germany.
JuStruct: Jülich Center for Structural Biology, Forschungszentrum Jülich, 52428 Jülich, Germany.
Research Center for Mechanisms of Aging and Age Related Diseases, Moscow Institute of Physics and Technology (National Research University), Dolgoprudny 141701, Russia.
Institute of Crystallography, University of Aachen (Rheinisch-Westfälische Technische Hochschule Aachen [RWTH]), 52062 Aachen, Germany.

I Gushchin (I)

Research Center for Mechanisms of Aging and Age Related Diseases, Moscow Institute of Physics and Technology (National Research University), Dolgoprudny 141701, Russia.

J M Haro-Moreno (JM)

Evolutionary Genomics Group, Departamento de Producción Vegetal y Microbiología, Universidad Miguel Hernández, 03202 San Juan de Alicante, Spain.

I Chizhov (I)

Institute for Biophysical Chemistry, Hannover Medical School, 30625 Hannover, Germany.

S Siletsky (S)

Belozersky Institute of Physical-Chemical Biology, Lomonosov Moscow State University, Moscow 119234, Russia.

M Mamedov (M)

Belozersky Institute of Physical-Chemical Biology, Lomonosov Moscow State University, Moscow 119234, Russia.

A Rogachev (A)

Research Center for Mechanisms of Aging and Age Related Diseases, Moscow Institute of Physics and Technology (National Research University), Dolgoprudny 141701, Russia.
Frank Laboratory of Neutron Physics, Joint Institute for Nuclear Research, Dubna 141980, Russia.

T Balandin (T)

Institute of Biological Information Processing (Institute of Biological Information Processing: Structural Biochemistry), Forschungszentrum Jülich, 52428 Jülich, Germany.
JuStruct: Jülich Center for Structural Biology, Forschungszentrum Jülich, 52428 Jülich, Germany.

V Borshchevskiy (V)

Research Center for Mechanisms of Aging and Age Related Diseases, Moscow Institute of Physics and Technology (National Research University), Dolgoprudny 141701, Russia.

A Popov (A)

Structural Biology Group, European Synchrotron Radiation Facility, 38000 Grenoble, France.

G Bourenkov (G)

Hamburg Unit care of Deutsches Elektronen-Synchrotron (DESY), European Molecular Biology Laboratory, 22607 Hamburg, Germany.

E Bamberg (E)

Research Center for Mechanisms of Aging and Age Related Diseases, Moscow Institute of Physics and Technology (National Research University), Dolgoprudny 141701, Russia.
Biophysical Chemistry, Max Planck Institute of Biophysics, 60438 Frankfurt am Main, Germany.

F Rodriguez-Valera (F)

Research Center for Mechanisms of Aging and Age Related Diseases, Moscow Institute of Physics and Technology (National Research University), Dolgoprudny 141701, Russia.
Evolutionary Genomics Group, Departamento de Producción Vegetal y Microbiología, Universidad Miguel Hernández, 03202 San Juan de Alicante, Spain.

G Büldt (G)

Research Center for Mechanisms of Aging and Age Related Diseases, Moscow Institute of Physics and Technology (National Research University), Dolgoprudny 141701, Russia.

V Gordeliy (V)

Institut de Biologie Structurale J.-P. Ebel, Université Grenoble Alpes-Commission for Atomic Energy (CEA)-CNRS, 38000 Grenoble, France; valentin.gordeliy@ibs.fr.
Institute of Biological Information Processing (Institute of Biological Information Processing: Structural Biochemistry), Forschungszentrum Jülich, 52428 Jülich, Germany.
JuStruct: Jülich Center for Structural Biology, Forschungszentrum Jülich, 52428 Jülich, Germany.
Research Center for Mechanisms of Aging and Age Related Diseases, Moscow Institute of Physics and Technology (National Research University), Dolgoprudny 141701, Russia.

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Classifications MeSH

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