Disturbed expression of autophagy genes in blood of Parkinson's disease patients.
Adaptor Proteins, Signal Transducing
/ genetics
Aged
Aged, 80 and over
Apoptosis Regulatory Proteins
/ genetics
Autophagy
/ genetics
Biomarkers
/ blood
Dopaminergic Neurons
/ metabolism
Female
France
Gene Expression
/ genetics
Gene Expression Profiling
/ methods
Gene Expression Regulation
/ genetics
Humans
Leukocytes, Mononuclear
Machine Learning
Male
Microtubule-Associated Proteins
/ genetics
Middle Aged
Parkinson Disease
/ blood
Pilot Projects
Sequestosome-1 Protein
/ genetics
Autophagy genes
Blood cells
Differential gene expression
Droplet Digital PCR
Parkinson’s disease
Journal
Gene
ISSN: 1879-0038
Titre abrégé: Gene
Pays: Netherlands
ID NLM: 7706761
Informations de publication
Date de publication:
15 May 2020
15 May 2020
Historique:
received:
03
09
2019
revised:
17
01
2020
accepted:
04
02
2020
pubmed:
9
2
2020
medline:
28
4
2020
entrez:
9
2
2020
Statut:
ppublish
Résumé
Parkinson's disease (PD) is a common neurodegenerative disorder which affects dopaminergic neurons leading to alteration of numerous cellular pathways. Several reports highlight that PD disturbs also other cells than CNS neurons including PBMCs, which could lead, among other things, to dysfunctions of immune functions. Because autophagy could be altered in PD, a monocentric pilot study was performed to quantify the transcripts levels of several autophagy genes in blood cells. MAP1LC3B, GABARAP, GABARAPL1, GABARAPL2 and P62/SQSTM1 were found to be overexpressed in patients. On the contrary, transcripts for HSPA8 and GAPDH were both decreased. Expression of MAP1LC3B and GABARAP was able to successfully segregate PD patients from healthy controls. The accuracy of this segregation was substantially increased when combined expressions of MAP1LC3B and GAPDH or GABARAP and GAPDH were used as categorical variables. This pilot study suggests that autophagy genes expression is dysregulated in PD patients and may open new perspectives for the characterisation of prediction markers.
Identifiants
pubmed: 32035240
pii: S0378-1119(20)30123-2
doi: 10.1016/j.gene.2020.144454
pii:
doi:
Substances chimiques
Adaptor Proteins, Signal Transducing
0
Apoptosis Regulatory Proteins
0
Biomarkers
0
GABARAP protein, human
0
GABARAPL1 protein, human
0
MAP1LC3B protein, human
0
Microtubule-Associated Proteins
0
SQSTM1 protein, human
0
Sequestosome-1 Protein
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
144454Informations de copyright
Copyright © 2020 Elsevier B.V. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.