A programmable microfluidic platform for multisample injection, discretization, and droplet manipulation.
Journal
Biomicrofluidics
ISSN: 1932-1058
Titre abrégé: Biomicrofluidics
Pays: United States
ID NLM: 101293825
Informations de publication
Date de publication:
Jan 2020
Jan 2020
Historique:
received:
26
12
2019
accepted:
26
01
2020
entrez:
11
2
2020
pubmed:
11
2
2020
medline:
11
2
2020
Statut:
epublish
Résumé
A programmable microfluidic platform enabling on-demand sampling, compartmentalization, and manipulation of multiple aqueous volumes is presented. The system provides random-access actuation of a microtrap array supporting selective discretization of picoliter volumes from multiple sample inputs. The platform comprises two interconnected chips, with parallel T-junctions and multiplexed microvalves within one chip enabling programmable injection of aqueous sample plugs, and nanoliter volumes transferred to a second microtrap array chip in which the plugs are actively discretized into picoliter droplets within a static array of membrane displacement actuators. The system employs two different multiplexer designs that reduce the number of input signals required for both sample injection and discretization. This versatile droplet-based technology offers flexible sample workflows and functionalities for the formation and manipulation of heterogeneous picoliter droplets, with particular utility for applications in biochemical synthesis and cell-based assays requiring flexible and programmable operation of parallel and multistep droplet processes. The platform is used here for the selective encapsulation of differentially labeled cells within a discrete droplet array.
Identifiants
pubmed: 32038741
doi: 10.1063/1.5143434
pii: 1.5143434
pmc: PMC7002170
doi:
Types de publication
Journal Article
Langues
eng
Pagination
014112Informations de copyright
Copyright © 2020 Author(s).
Références
Biomicrofluidics. 2014 Jun 25;8(3):034118
pubmed: 25379078
Lab Chip. 2008 Feb;8(2):198-220
pubmed: 18231657
Lab Chip. 2016 May 21;16(10):1909-16
pubmed: 27102263
Proc Natl Acad Sci U S A. 2008 Mar 4;105(9):3191-6
pubmed: 18316742
Lab Chip. 2016 Jul 7;16(13):2474-80
pubmed: 27250530
Lab Chip. 2006 Aug;6(8):1073-9
pubmed: 16874381
High Throughput. 2018 Jun 20;7(2):
pubmed: 29925804
Lab Chip. 2016 Dec 20;17(1):34-75
pubmed: 27841886
Anal Chem. 2010 Jul 1;82(13):5707-17
pubmed: 20550137
Rep Prog Phys. 2012 Jan;75(1):016601
pubmed: 22790308
Lab Chip. 2015 Sep 7;15(17):3439-59
pubmed: 26226550
Biomicrofluidics. 2012 Mar;6(1):14110-1411013
pubmed: 22662095
Proc Natl Acad Sci U S A. 2012 May 15;109(20):7665-70
pubmed: 22547789
Lab Chip. 2008 Jul;8(7):1110-5
pubmed: 18584086
Microfluid Nanofluidics. 2013 Jul 1;15(1):117-126
pubmed: 23935562
Chem Rev. 2017 Jun 28;117(12):7964-8040
pubmed: 28537383
Lab Chip. 2013 Nov 21;13(22):4343-51
pubmed: 24056989
Wiley Interdiscip Rev Nanomed Nanobiotechnol. 2018 Nov;10(6):e1522
pubmed: 29797414
Proc Natl Acad Sci U S A. 2007 Jan 23;104(4):1146-51
pubmed: 17227861
Lab Chip. 2006 Mar;6(3):437-46
pubmed: 16511628
Lab Chip. 2019 Jan 29;19(3):524-535
pubmed: 30633267
Conf Proc IEEE Eng Med Biol Soc. 2018 Jul;2018:5350-5353
pubmed: 30441545
Lab Chip. 2013 May 7;13(9):1817-22
pubmed: 23507976
Lab Chip. 2009 Mar 7;9(5):692-8
pubmed: 19224019
Soft Matter. 2015 May 21;11(19):3884-99
pubmed: 25864524
Nat Protoc. 2013 May;8(5):870-91
pubmed: 23558786
Lab Chip. 2017 Oct 25;17(21):3717-3724
pubmed: 28990023
Lab Chip. 2009 Jan 21;9(2):331-8
pubmed: 19107293
Biomicrofluidics. 2013 Nov 06;7(6):64102
pubmed: 24396536
Lab Chip. 2010 Nov 7;10(21):2855-63
pubmed: 20882233
Lab Chip. 2016 Apr 26;16(9):1698-707
pubmed: 27075732
Annu Rev Biophys. 2010;39:139-58
pubmed: 20192773
Lab Chip. 2009 Feb 21;9(4):516-20
pubmed: 19190786
Proc Natl Acad Sci U S A. 2009 Aug 25;106(34):14195-200
pubmed: 19617544
Lab Chip. 2017 Oct 11;17(20):3422-3430
pubmed: 28792054
Annu Rev Biophys Biomol Struct. 2007;36:213-31
pubmed: 17269901
Lab Chip. 2008 Aug;8(8):1262-4
pubmed: 18651066
Lab Chip. 2009 May 7;9(9):1298-300
pubmed: 19370253
Lab Chip. 2009 Jun 7;9(11):1504-6
pubmed: 19458854
J Chromatogr A. 2003 Sep 26;1013(1-2):29-33
pubmed: 14604105
Lab Chip. 2019 Jan 29;19(3):493-502
pubmed: 30623951
Lab Chip. 2008 Feb;8(2):259-66
pubmed: 18231664
Anal Chem. 2011 Mar 1;83(5):1603-8
pubmed: 21280615
Science. 2002 Oct 18;298(5593):580-4
pubmed: 12351675