Sensitivity-enhanced three-dimensional and carbon-detected two-dimensional NMR of proteins using hyperpolarized water.
3D NMR
BEST-HNCO
Direct 13C detection
Dissolution-dynamic nuclear polarization (D-DNP)
Hyperpolarization
Non-uniform sampling
Journal
Journal of biomolecular NMR
ISSN: 1573-5001
Titre abrégé: J Biomol NMR
Pays: Netherlands
ID NLM: 9110829
Informations de publication
Date de publication:
Mar 2020
Mar 2020
Historique:
received:
09
09
2019
accepted:
27
01
2020
pubmed:
11
2
2020
medline:
9
10
2020
entrez:
11
2
2020
Statut:
ppublish
Résumé
Signal enhancements of up to two orders of magnitude in protein NMR can be achieved by employing HDO as a vector to introduce hyperpolarization into folded or intrinsically disordered proteins. In this approach, hyperpolarized HDO produced by dissolution-dynamic nuclear polarization (D-DNP) is mixed with a protein solution waiting in a high-field NMR spectrometer, whereupon amide proton exchange and nuclear Overhauser effects (NOE) transfer hyperpolarization to the protein and enable acquisition of a signal-enhanced high-resolution spectrum. To date, the use of this strategy has been limited to 1D and
Identifiants
pubmed: 32040802
doi: 10.1007/s10858-020-00301-5
pii: 10.1007/s10858-020-00301-5
pmc: PMC7080779
doi:
Substances chimiques
Intrinsically Disordered Proteins
0
SPP1 protein, human
0
Ubiquitin
0
Water
059QF0KO0R
Osteopontin
106441-73-0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
161-171Subventions
Organisme : European Research Council
ID : 339754
Pays : International
Organisme : European Research Council
ID : 279519
Pays : International
Organisme : European Research Council
ID : 801936
Pays : International
Organisme : Israel Science Foundation
ID : 965/18
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