Precise timing of transcription by c-di-GMP coordinates cell cycle and morphogenesis in Caulobacter.
Bacterial Proteins
/ genetics
Caulobacter crescentus
/ cytology
Cell Cycle
/ genetics
Cyclic GMP
/ analogs & derivatives
Gene Expression Regulation, Bacterial
Histidine Kinase
/ chemistry
Morphogenesis
/ genetics
Phosphorylation
Protein Binding
Protein Domains
Proteolysis
Signal Transduction
Trans-Activators
/ genetics
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
10 02 2020
10 02 2020
Historique:
received:
29
06
2019
accepted:
13
01
2020
entrez:
12
2
2020
pubmed:
12
2
2020
medline:
28
4
2020
Statut:
epublish
Résumé
Bacteria adapt their growth rate to their metabolic status and environmental conditions by modulating the length of their G1 period. Here we demonstrate that a gradual increase in the concentration of the second messenger c-di-GMP determines precise gene expression during G1/S transition in Caulobacter crescentus. We show that c-di-GMP stimulates the kinase ShkA by binding to its central pseudo-receiver domain, activates the TacA transcription factor, and initiates a G1/S-specific transcription program leading to cell morphogenesis and S-phase entry. Activation of the ShkA-dependent genetic program causes c-di-GMP to reach peak levels, which triggers S-phase entry and promotes proteolysis of ShkA and TacA. Thus, a gradual increase of c-di-GMP results in precise control of ShkA-TacA activity, enabling G1/S-specific gene expression that coordinates cell cycle and morphogenesis.
Identifiants
pubmed: 32041947
doi: 10.1038/s41467-020-14585-6
pii: 10.1038/s41467-020-14585-6
pmc: PMC7010744
doi:
Substances chimiques
Bacterial Proteins
0
PleD protein, Caulobacter crescentus
0
TacA protein, Caulobacter crescentus
0
Trans-Activators
0
bis(3',5')-cyclic diguanylic acid
61093-23-0
Histidine Kinase
EC 2.7.13.1
Cyclic GMP
H2D2X058MU
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
816Références
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